Mohammad Shokrzadeh - Department of Pharmacology and Toxicology, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Mohammad Azadbakht - Department of Pharmacognosy, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Hamidreza Shakibamanesh - Student Research Committee Pharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

Arnebia euchroma is from Boraginaceae family. Various therapeutic effects have been reported for different species of this plant. The aim of this study was to investigate antioxidant activity of hydroalcoholic extract of the root of A. euchroma and its protective effect on hepatotoxicity induced by CCl۴ in mice. In this experimental study, A. euchroma was extracted with ethanol solvent by maceration method. Antioxidant activity of the plant has been evaluated by the DPPH free radical scavenging method. Then, the hepatoprotective effect of A. euchroma was evaluated through determination the content of glutathione in liver tissue, ALP, AST, ALT serum levels and histopathology of liver in different groups. The total phenolic content determined as ۰.۵۶% per ۱۰۰ g of plant was equivalent to gallic acid. The total flavonoid content was ۰.۰۷۹% per ۱۰۰ g of plant that was equivalent to quercetin. The IC۵۰ of the extract for free radical scavenging was obtained ۱۳۹.۲ µg/ml. The extract increased liver glutathione and serums ALP, AST, ALT that were decreased by CCl۴ administration in animal. All protective effects were dose-dependent and the results of liver histopathology showed a significant improvement of inflammation and necrosis that were caused by CCl۴ in mice. The results of this study revealed that the hydroalcoholic extract of A. euchroma root has an antioxidant effect and excellent hepatoprotection in mice.

Hepatoprotective, arnebia euchroma, CCl۴, mice