Hossein Danafar - Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran

Cefixime is essential member of orally energetic third generation cephalosporin and has tremendous activity aligned with many pathogens. The virtual bioavailability of a newly industrial dispersible tablet as compared with a recognized identified formulation.  A simple and available reversed-phase HPLC method with UV detection has been urbanized and validate for cefixime evaluate in human plasma using a C۱۸ analytical column and a mobile phase of tetrabutylammonium hydroxide (pH ۶.۵)-acetonitrile (۳:۱ v/v). The detection wavelength was ۲۸۰ nm. To method observed major linear response-concentration association all through the cefixime concentration range of ۱۵-۱۰۰ ng/ml, with the average accuracy within-run and between-run values of ۹۷.۲۹% and ۹۹.۲۷%. The average drug recovery from plasma was ۹۸.۲% throughout the linear concentration range. The limits of detection (LOD) and quantitation (LOQ) of the method were ۵ and ۱۵ ng/ml, respectively. The method is quick, easy, very steady and precise for the partition, assignment, evaluation of cefixime in human plasma.

Cefixime, human plasma, reversed-phase HPLC