Mahmoud Roushani - Department of Chemistry, Ilam University, P. O. Box: ۶۹۳۱۵-۵۱۶, Ilam, Iran
Masoumeh Sarabaegi - Department of Chemistry, Ilam University, P. O. Box: ۶۹۳۱۵-۵۱۶, Ilam, Iran
Hadi Hosseini - Department of Chemistry, Ilam University, P. O. Box: ۶۹۳۱۵-۵۱۶, Ilam, Iran

The combination of aptamer is performed with molecularly imprinted (MIP) using their unique features. In this sense, first the screen-printed carbon electrode (SPCE) was modified with nano size iridium cupper on nitrogen-doped carbon nanocapsoul (CN@IrCu) and, then, the Aptamer -MIP hybrid was used to detect Helicobacter pylori. This design leads to the use of dual properties of Aptamer and MIP. Moreover, CN@IrCu has been used as a substrate to increase aptamer loading and the conductivity. The sensing surface fabrication steps was measured by several different electrochemical techniques such as cyclic voltammetric (CV), differential pulse voltammetric (DPV), electrochemical impedance spectroscopy (EIS). The sensor shows a linear range from ۱۰۲ CFU.ml-۱ to ۱۰۷ CFU.ml-۱ with a detection limit of ۳۳ CFU.ml-۱. This Aptamer-MIP/CN@IrCu/SPCE was successfully evaluated in determining the concentration of Helicobacter pylori in blood serum samples with high reproducibility and selectivity. This MIP-aptasensor is low cost, low response time, high stability and good sensitivity.

Molecularly imprinted polymers, Screen Printed Carbon Electrode, Aptamer, Helicobacter pylori, Aptamer-MIP