Camellia Taghadosi - Graduated student, Faculty of Veterinary Medicine, Shahrekord university, Shahrekord, Iran.
Gholamali Kojouri - Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord university
Alimohammad Ahadi - Assistant Professor, Department of Genetic, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.
Mohammad Hashemi Bahremani - Associate Professor, Laboratory of Pathology, Emam Hosein Hospital, Tehran, Iran.
Abdolnabi Kojouri - Faculty of Medicine, International Campus of Shahid Sadoughi Medical University, Yazd, Iran

Background: Bovine leukaemia virus (BLV) is the cause of enzootic bovine leukaemia which belongs to retroviruses including Human T-cell leukaemia virus and simian T-lymphotropic virus. Due to this familiarity, the possibility of virus (BLV) transfer from animal production to humans may exist. Materials and methods: In the present study, formalin-fixed, paraffin-embedded and fresh human lymphomas tissues were used for detecting the BLV Tax genome and BLV Tax expression using nested-PCR and RT-nested-PCR. Results: Nested-PCR evaluation showed that ۹ of ۴۱ samples contained Tax region of BLV genome, of which eight samples belonged to high grade diffuse large cell lymphoma (B-cell type). Also, the results of RT-nested-PCR showed the BLV Tax expression in ۲ of ۵ high grade diffuse large cell lymphoma (B-cell type) samples. Conclusion: These findings explain the possible relation between BLV infection and the occurrence of some types of human lymphomas for the first time.

BLV, lymphoma, nested PCR, RT-nested-PCR, Tax.