Zahra Tahmaseni Fard - Assistant Professor of cellular and Molecular Biology, Roudehen Branch, Islamic Azad University, Roudehen, IRAN.
Mandana Hasanzad - Medical Genomics Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Mohammad Reza Nowroozi - Uro Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Hanieh zham - Department of pathology , Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IRAN & Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN

Background: By binding SHBG hormone to sex hormones, in addition to carrying them in the blood, they regulate the amount of tissue availability. Since the genetic changes in the structure of globulin affect it’s binding to hormones, so in this study the effects of single nucleotide change in exon ۸ or rs ۶۲۵۹ in the incidence of prostate cancer is evaluated. Methods: The study population included ۱۲۰ patients with prostate cancer and ۱۲۰ control subjects. After collecting blood samples, DNA was extracted by salting out the method in order to determine the genotype of individuals by RFLP-PCR method. According to Hardy-Weinberg equilibrium genotypes and allele frequencies were calculated and a relationship between this variation and prostate cancer were evaluated and by using SPSS ۲۳ software and the relationship between variations. The significance level was considered ≤۰.۰۵. Results: Results indicated that homozygous mutant genotype AA ۲.۵۸ (p value= ۰.۰۰۷, OR: ۲.۵۸, CI۹۵%: ۱.۵۲-۴.۳۸) and heterozygous AG ۱.۱۸ times (p-value =۰.۵, OR: ۱.۱۸, CI۹۵%: ۰.۳۸-۳.۶۱) increase chance of getting prostate cancer in carriers. But homozygous of wild genotype GG have the protective role against prostate cancer (p-value =۰.۰۰۵, OR: ۰.۳۸۵, CI۹۵%: ۰.۲۳-۰.۶۵). Conclusion: Therefore, Asn allele is one of the main factors in prostate cancer so it can be used as the non-invasive and suitable marker for early detection in susceptible individuals.

Prostate cancer, Sex hormone-binding globulin, PCR-RFLP