Generation of CHO Stable Cell Line Overexpressing HER۲: an In Vitro Model for Breast Cancer

Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_REMJ-3-3_002

تاریخ نمایه سازی: 22 دی 1402

Abstract:

Background: Breast cancer is the most common female malignancy and the leading cause of cancer mortality in women worldwide. The human epidermal growth factor receptor۲ (HER۲) is a transmembrane tyrosine kinase receptor that is usually overexpressed in human breast cancers. Stable cell lines heterogeneously overexpressing HER۲ are highly required as in vitro models for breast cancer research. The aim of this study was to establish a stable cell line overexpressing HER۲. Materials and Methods: CHO Cells were transfected with linearized pCVN/HER۲ plasmid and selected for the recombinant cells with G۴۱۸ antibiotic. Expression of HER۲ in the transfected cells was analyzed using western blotting and immunofluorescence. Results: We found that the recombinant cells stably expressed high levels of HER۲ proteins that were mostly concentrated on the cell membrane. Conclusions: The cell line established here provides a useful in vitro model for breast cancer research and any HER-related studies.

Authors

Akbar Hedayatizadeh-Omran

Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

Reza Valadan

Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

Alireza Rafiei

Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Abolghasem Ajami

Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Mohsen Tehrani

Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Reza Alizadeh-Navaei

Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran

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