Detection of CYP۲C۱۸ m۱, and m۲ Alleles within an Iranian Population (Mazandaran) Using Denaturing High-Performance Liquid Chromatography (DHPLC)

Background: Genetic polymorphisms of cytochrome p۴۵۰ in humans are the main cause of differences in the metabolism. The allele and genotype frequencies of CYP۲C۱۹ and CYP۲C۹ have been studied in some Iranian populations. The aim of present study was to examine the frequencies of CYP۲C۱۸m۱, and CYP۲C۱۸m۲, alleles in the Mazandarani ethnic group among Iranian Population. Materials and Methods: In this study, genomic DNA was extracted from leucocytes of one hundred unrelated healthy volunteers. The prevalence of the common variants CYP۲C۱۸ m۱ and m۲ alleles were studied by using high fidelity polymerase chain reaction (HF-PCR) - DHPLC methods. Results: The frequency of CYP۲C۱۸ m۱ and m۲ alleles were ۰.۰% and ۳.۰%, respectively. CYP۲C۱۸ genotypes wt/wt, wt/m۱, wt/m۲, m۱/m۱, m۱/m۲, and m۲/m۲ frequencies were ۹۷ %, ۰.۰ %, ۳.۰%, ۰.۰ %, ۰.۰%, and ۰.۰%, respectively. Conclusion: The result of the current study shows that impaired CYP۲C۱۸ activity in ۰۳% of our sample population may decrease extra hepatic metabolism of some important drugs such as Phenytoin. It may also affect transdemal delivery of drug substrate for this isoenzyme.

Keywords:

CYP۲C۱۸ , Genotype , Alleles , Mazandaran , Iranian