Desalegn Akuma - Environmental Engineering, Jimma Technology Institute, Jimma University, Jimma City, Ethiopia
Ketema Hundie - Process Engineering, Jimma Technology Institute, Jimma University, Jimma City, Ethiopia
Abraham Bayu - Environmental Engineering, Jimma Technology Institute, Jimma University, Jimma City, Ethiopia

Plants produce an extensive range of phytochemical components which are secondary metabolites. Phytochemicals have the ability to act as antioxidants by preventing cell damages which is usually caused by free radicals such as those associated with heart disease and cancer. Neem seed have high potential of phytochemicals, which uses as traditional medicinal purpose. The antimicrobial activities of Neem seed crude bioactive extracted with distilled water examined. Evaluating Neem seed bioactive extract phytochemical was the objective of this study. Neem seed bioactive components were Examined by the means of agar well diffusion method. Tannins, Quinones and phenols were the highest phytochemical in Neem seed bioactive extract, Anthocyanin, Terpenoids, Saponins and Glycosides were in medium levels and while Alkaloids and Flavonoids were in low level concentration. At a lowest concentration of bioactive (۱۰mg/mL), Bacillus subtilis (G+) was the best controllable compared to other. The optimum concentration for selected pathogenic species was ۵۵mg/mL and at this concentration, Salmonella typhimurium (G-) was highly controllable which ۴۱mm control diameter. Staphylococcus aureus (G+), Bacillus subtilis (G+), and Escherichia coli (G-) were ۴۰mm, ۳۸mm and ۳۴mm control in diameter respectively.

antibacterial, bioactive extract, Pathogenic bacteria, optimum concentration, Neem Seed