S Mohammadi - MSc, Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
E Soratijahromi - MSc, Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
R Dehdari Vais - MSc, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
N Sattarahmady - PhD, Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Background: As an alternative form of cancer therapy, photothermal therapy (PTT) and sonodynamic therapy (SDT) using nanomaterials are in development. Nanomaterials can act as energy absorber as well as anti-cancer agent. Objective: In this study, the effects of laser and ultrasound irradiation with Se-PEG-Cur nanoparticles were investigated on melanoma cancer.Material and Methods: In this experimental study, nanoparticles of selenium-polyethylene glycol-curcumin (Se-PEG-Cur) were synthesized, and their UV-vis absorption, particle size, zeta potential and photothermal conversion efficiency were determined. Se-PEG-Cur was then introduced as a novel ۸۰۸-nm laser light absorbing agent as well as ultrasound (US) wave for treatment of C۵۴۰ (B۱۶/F۱۰) cancer cells. Also, ROS generation in C۵۴۰ (B۱۶/F۱۰) cancer cells was measured upon PTT and SDT using Se-PEG-Cur. Results: Mean size, zeta potential and photothermal conversion efficiency of Se-PEG-Cur were obtained as ~۳۰۰ nm, ۴۲.۷ mV and ۱۶.۷%, respectively. Cell viability upon irradiation of the laser light or US waves with ۱۰۰ µg mL-۱ Se-PEG-Cur were decreased to ۳۳.۹ and ۲۲.۹%, respectively. Conclusion: Intracellular ROS detection indicated that dual PTT and SDT in the presence of Se-PEG-Cur induced the highest ROS production. Se-PEG-Cur was therefore introduced as an absorbing agent of both laser light and US waves for cancer treatment.

Hyperthermia, Skin Cancer, Diode Laser, Ultrasound, Nanomedicine, Reactive Oxygen Species, Cell Survival