M Haghani - PhD, Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
V Pouladvand - MSc, Department of Biochemical, Jiroft University of Medical Sciences, Jiroft, Kerman, Iran
S M J Mortazavi - PhD, Ionizing and Non-Ionizing Radiation Protection Research Center, Paramedical School, Shiraz University of Medical Sciences, Shiraz, Iran
M Razavinasab - PhD, Kerman Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran
M Bayat - PhD, Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
M Shabani - PhD, Kerman Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran

Background: Prenatal adverse effects of radiofrequency electromagnetic fields (RF-EMF) exposure on nervous system are an issue of major concern.Objective: Thus, in this study we evaluated the membrane current flow properties of Purkinje neurons after maternal exposure to ۹۰۰ MHz pulsed RF-EMF.Material and Methods: In this experimental study, during all days of pregnancy, rats in the EMF-exposed group were exposed to ۹۰۰ MHz pulsed-EMF radiation for ۶ h per day. The effects of RF-EMF exposure on the electrophysiological properties of the Purkinje cerebellum neurons from male pups were evaluated by whole-cell patch clamp recordings in current and voltage clamp modes. In voltage-clamp experiments, the holding potential was -۶۰ mV, and a depolarizing voltage step (۱۰۰۰ ms duration) was applied from -۶۰ to +۵۰ mV in ۱۰ mV increments at ۲s intervals. Results: The exposure group demonstrated reduced spontaneous firing associated with upward and rightward shift in I/V curve compared to the control rats. Moreover, the peak amplitude of the current for the exposure pups also revealed a significant decrement. The reversal potential was +۴۰ mV and +۲۰ mV for the control and RF-EMF groups, respectively and showed significant differences between the two groups. Conclusion: The decrease in ion’s conductance could be attributed to the observed decrease in the voltage onset of the inward current, peak amplitude and voltage shift.

Electrophysiological Properties, Cell Phone, Purkinje cells, Prenatal Injuries