Radiosensitization of Glioma Cells by Temozolomide (TMZ): A Colony Formation Assay

Background: Glioblastoma is one of the most radioresistant cancers. It is suggested that combination of radiotherapy with other cancer treatment modalities may increase control of tumor. Temozolomide (TMZ) is one of the most known drugs for glioblastoma. It has shown that TMZ via induction of mutation and cell death can kill glioma cells. Objective: In the current study, we aimed to show possible radiosensitization effect of TMZ for glioma cells. In addition, results compared to response of normal fibroblast cells to TMZ and irradiation.Material and Methods: This is in vitro study for evaluation of the effect of TMZ and irradiation on high grade glioma cells and normal fibroblasts. The human fibroblast and glioma cells were cultured as monolayer. The cells were treated with ۲۰۰۰ μM TMZ, which was equal drug dose for IC۵۰%. In addition, irradiation was done with ۵Gy gamma rays. The formation of colony was observed following irradiation, treatment with TMZ, and combination both of them. Results: The formation of colony for both glioma and fibroblast cells showed a reduction following irradiation or treatment with TMZ. Irradiation showed more toxicity compared to TMZ for glioma cells, but not fibroblast cells. Combination of TMZ and irradiation showed a significant reduction in the colony formation compared to irradiation or TMZ treatment alone. Conclusion: This study showed that TMZ increases sensitivity of both glioma and fibroblast cells to ionizing radiation.

Keywords:

Temozolomide , Radiation , Glioblastoma , Fibroblast Cells