Mostafa Aalipour - Department of immunology, Afzalipour faculty of medicine, Kerman University of Medical Sciences, Kerman, Iran.
Mohammad Javad Zahedi - Department of Internal Medicine, Kerman University of Medical Sciences, Kerman, Iran
Mohammad Reza Zangouey - Department of immunology, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Abolfazl Yari - Cellular and Molecular research center. Birjand University of Medical Sciences, Birjand, Iran.
Moghaddameh Mirzaee - Department of Biostatistics and Epidemiology, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran
Mohammad Mahdi Mohammadi - Department of immunology, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

Background: Irritable bowel syndrome is a functional gastrointestinal disease of unknown etiology. Researchers have recently drawn attention to the possible role of viruses in the development of IBS and provided evidence in this regard. In this study, it was decided to investigate the possible role of rotavirus infection in the onset of IBS.Methods: Stool and serum samples were collected from ۴۰ patients with IBS and ۴۰ healthy individuals. To evaluate the previous exposure to rotavirus we checked the presence and concentration of anti-rotavirus IgG by ELISA. ELISA test was performed on the serum samples. Real-time PCR test was also used to measure the viral load in the stool. Finally, the data were analyzed by SPSS-۲۲ software.Results: No significant relationship was found between anti-rotavirus IgG presence and Level in the serum of case and healthy individuals (p-value > ۰.۰۵) . Moreover, there was no significant difference between the viral genome load in the stool samples of the two groups (p-value > ۰.۰۵).Conclusion: According to the results, it seems unlikely that a link exists between rotavirus infection and the onset of irritable bowel syndrome, but the possible role of other gastrointestinal viruses, including coronavirus, remains.

Irritable bowel syndrome, Rotavirus, Pattern Recognition Receptors, Coronavirus