Sara Daneshmand - Department of pharmaceutics, faculty of pharmacy, Zabol University of Medical Sciences, Zabol, Iran
Mitra Niazi - Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Bahman Fazeli-Nasab - Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran
Javad Asili - Department of pharmacognosy, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Shiva Golmohammadzadeh - Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
R. Z. Sayyed - Department of Microbiology, PSGVP Mandal’s S I Patil Arts, G B Patel Science and STKV Sangh Commerce College, Shahada ۴۲۵۴۰۹, India

Hair loss and hirsutism have been major complaints due to increased concentrations of dihydrotestosterone. The plant Platycladus orientalis, with ۵-alpha reductase inhibitor properties, has been used to treat these disorders. Its formulation with lipophilic carriers in SLN possesses high loading capacity and greater permeability to hair follicles. The present study aimed to determine the content of active ingredients in the extract of P. orientalis L. and to prepare and characterize the solid lipid nanoparticles (SLN) of Platycladus orientalis L. extract as a ۵-alpha reductase inhibitor. The total methanolic extract was obtained following the maceration technique. This preparation was analyzed by HPLC using Quercetin and Cedrol as standard components. SLNs were prepared by high-shear homogenization and ultrasound. Four Glucire-GMS-Compritol-Precirol lipids and three poloxamer-tween۸۰-Labrasol surfactants were further used in the formulations. Particle size, zeta potential, nanoparticle morphology, encapsulation percentage, crystal structure, physical stability, size, and zeta potential were studied ۰, ۳, and ۶ months after preparation. Within ۱-۷ days after preparation, formulations containing GMS and compritol lipids became solid and jelly. Meanwhile, the formulations with Precirol as the lipid and Poloxamer as the surfactant with ۰.۳% extract exhibited desirable properties such as average particle size (۱۹۲ nm), the encapsulation of the extract inside the nanoparticles was almost ۷۱%, and good zeta potential. This formulation containing precirol as a lipid, poloxamer as a surfactant, and ۰.۳% plant extract exhibited greater ۵-alpha reductase inhibitor activity, and it can be recommended to treat hair loss and hirsutism.

Cedrol, hair loss, hirsutism, HPLC, solid-lipid nanoparticles