Bahman Fazeli-Nasab - Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran
Mehrangiz Ghafari - Department of Pathology, School of Medicine, Zabol University of medical sciences, Zabol, Iran
Mehdi Jahantigh - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
Zahra Beigomi - Zahedan University of Medical Sciences, Zahedan, Iran
Saeide Saeidi - Agricultural Biotechnology Research Institute, University of Zabol, Zabol, Iran

In general, the resistance of microorganisms to antibiotics has become one of the major concerns of human society and the health care system. The aim of this study was to evaluate total phenol, flavonoids content, alkaloids, antioxidant capacity and antibacterial activity of ۷ medicinal plants extracts in Sistan climatic conditions against pathogens. Plant samples were collected from the collection of the Agricultural Research Institute of medicinal plants, University of Zabol and dried in normal shade and room temperature. A Methanolic extract of the leaves of snake grass, eucalyptus, tatura, Musquit bean, Watercress, rosemary and olive was prepared by cold maceration method. Total phenol content by using folin-ciocalteu reagent method, total flavonoid content by aluminum chloride colorimetric method, antioxidant capacity by DPPH free radical scavenging assay and antibacterial activity of extracts by agar diffusion method and measurement of growth inhibition zone or Disk diffusion was measured. The analysis of variance showed that there was a statistically significant difference between different medicinal plants at a probability level of ۵%. In the present experiment, the highest phenolic content (۱۱۰.۷۸ mg/q D.W.) in the Rosemary methanolic extract, the highest total flavonoid content (۴.۵۵ mg GA/g D.W.) in Eucalyptus extract and the highest antioxidant activity (۹۳.۱%) in the Olive extract were observed. Also, the largest diameter of Disk diffusion (۲۵ mm) was observed in the medium containing Rosemary extract and against Streptococcus pyogenes.

rosemary, DPPH, Olive, Streptococcus pyogenes, Streptococcus pneumoniae