Narjes Rezaei
Zahra Zaherijamil
Shirin Moradkhani
Massoud Saidijam
Iraj khodadadi
Ebrahim Abbasi Oshaghi
Heidar Tavilani

Background: It is shown that kiwifruit elevates serum high-density lipoprotein cholesterol (HDL-C) levels and exhibits beneficial effects on human health due to its antioxidant potential. Objectives: This study aimed to investigate the impact of kiwifruit on the activity of the paraoxonase ۱ (PON۱) enzyme, as a main antioxidant enzyme in HDL functionality, in a high-fat diet (HFD). Methods: To this end, ۴۲ male Syrian hamsters were divided into ۶ groups including hamsters receiving a normal diet (the control normal group), a regular diet supplemented with kiwifruit at two concentrations (i.e., ۱.۸۶ g/kg and ۳.۷۳ g/kg), a HFD comprised of ۱۵% butterfat + ۰.۰۵% cholesterol (the control high-fat group), and a HFD supplemented with kiwifruit at two concentrations (i.e., ۱.۸۶ and ۳.۷۳ g/kg) for ۸ weeks. Results: The results showed that supplementation of kiwifruit to the HFD increased the levels of HDL-C and remarkably reduced the concentrations of oxidized low-density lipoprotein (ox-LDL) and malondialdehyde (MDA) compared with the control-HF group. In addition, the paraoxonase activity of PON۱ significantly increased in HFD supplemented with kiwifruit (۱.۸۶ g/kg), and finally, arylesterase (ARE) activity increased in all treated groups when compared with untreated groups. Conclusion: Our findings suggested that kiwifruit can improve the lipid profile and prevent oxidative stress-induced by lipid peroxidation in hamsters receiving HFD, thus increasing the ARE and paraoxonase activities of PON۱.