Farima Jafari-Tirabadi
Shahin Hassanpour
Ahmad Asghari

Background: Previous reports suggest flavonoids as potent analgesic compounds. Objectives: Based on these observations, the present study investigated the anti-nociceptive action of troxerutin and neural interactions with opioidergic, serotoninergic, and nitrergic systems in mice. Methods: A total of ۳۴۰ male mice were randomly divided into ۲ categories. Each category included four experiments with four groups. In the first experiment of formalin examination, the animals intraperitoneally received saline and troxerutin (۵۰, ۱۵۰, and ۳۰۰ mg/kg). In the second experiment, the animals received saline, naloxone (۲ mg/kg), troxerutin (۳۰۰ mg/kg), and troxerutin+naloxone. In the third and fourth experiments, L-NAME (L-NG-Nitroarginine methyl ester) and cyproheptadine were injected. In this test, formalin was injected and paw licking time (pain sense) was recorded. In the writhing test, experimental groups were treated similarly and the mice were injected with acetic acid. Then, the inhibition of the writhing movements was recorded. Results: According to the findings, troxerutin decreased pain in the formalin test and writhing movements in the writhing test (P=۰.۰۰۱). Naloxone and troxerutin decreased licking time and writhing movements (P=۰.۰۰۱). L-NAME+troxerutin significantly diminished the anti-nociceptive effect of troxerutin on paw licking and inhibited pain response (P=۰.۰۰۱). Conclusion: These results suggested that troxerutin decreases inflammatory pain in mice, and this effect is mediated by opioidergic and nitrergic systems.