Adelina Simamora
Adit Widodo Santoso
Ika Rahayu
Kris Herawan Timotius

Introduction: Muntingia calabura is used for many medicinal advantages. So far, limited study has been done for the bioactivities of M.calabura fruit. The study aimed to investigate the enzyme inhibitory, antioxidant, and antibacterial activities of M.calabura fruit. Methods: Ethanol extract of M.calabura fruit was tested for its inhibitory enzyme activities against key enzymes linked to human pathologies, such as diabetes (α-glucosidase and α-amylase), hyperuricemia (xanthine oxidase), and obesity (lipase). The antioxidant properties were investigated using different in vitro assays (DPPH, CUPRAC, reducing power, phosphomolybdenum, metal chelating and DNA-Damage protection assays). The fruit was also evaluated for its antibacterial activity against several gram positive and negative bacteria. Results: The total phenolic and flavonoid contents of the extract were ۱۰.۸۵ mgGAE/g and ۳.۳۰ mg QE/g, respectively. The fruit extract showed good inhibition against α-glucosidase and α-amylase (IC۵۰ ۱۶.۷۴ and ۴۶.۴۹ µg/ml, respectively), with activities stronger than acarbose (۱۰۰.۳۸ and ۱۵۲.۴۶ µg/ml, respectively). It exhibited weak inhibitory activity against xanthine oxidase (IC۵۰ ۰.۹۱ mg/ml) and lipase (IC۵۰ ۱۶.۴۸ mg/ml), weaker than the references used for respective test (IC۵۰ allopurinol ۵.۳۱ µg/ml and orlistat ۰.۱۷ µg/ml). The extract showed antibacterial activities againts Chromobacterium violaceum, Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, and Escherichia coli. The ethanol extract showed weaker antioxidant activities, when compared to ascorbic acid and BHT. However, the extract was able to protect DNA-damage. Conclusions: The study concludes that M. calabura fruit exhibits antioxidant, antibacterial, and enzyme inhibitory properties, thus can be a good source for pharmacological uses.