Amit Gupta
Sushama R Chaphalkar

Introduction: Butea frondosa has been suggested to be very useful in treating inflammatory diseases but no scientific investigation has been done in such direction. In this study the anti-inflammatory and anti-microbial activities of leaves aqueous extract of B. frondosa were determined in infected and non-infected human whole blood against specific vaccine antigen, HBsAg.Methods: In order to explore the anti-inflammatory and anti-microbial activities of B. frondosa (۰.۵–۰ mg/mL; ۵۰ μl), infected (virally) and non infected (control) human whole blood samples were stimulated with hepatitis B vaccine containing surface antigen (HBsAg, ۲۰ μg/mL;۱۰ μl) in order to determined its blood counts and proliferation assay.Results: Aqueous leaves extract of B. frondosa (۱۰ mg/mL; ۵۰ μl) containing HBsAg inhibited the percentage count of monocytes as well as granulocytes population in both cases. In addition, this aqueous extract also reduced its proliferation rate at higher doses.Conclusion: Aqueous leaves extract of B. frondosa possesses both anti-inflammatory and antimicrobial activities and might be used for these purposes.