Jamal Gharekhani
Ehsan Barati
Ehsan Abbasi Doulatshahi
Mahdi Talaei
Mahmoud Reza Rasouli

Background: Domestic animals play a significant role in the distribution of Brucella infection to humans. Particularly, horses have a high impact on the transmission of zoonoses due to long life and multi-function. This project was conducted in Hamedan Veterinary Office for the primary assessment of Brucella infection in horses in Hamedan province using the serology technique. Methods: Between April ۲۰۱۹ and January ۲۰۱۹, a total of ۴۹۵ horse blood samples was collected from different equestrian clubs of Hamedan province. The animals were of different breeds without the clinical signs of disease. The samples were evaluated for the presence of anti-Brucella antibodies using the Rose-Bengal plate test (RBPT). Results: All animals were negative for Brucella antibodies. To the best of our knowledge, this is the first evaluation of Brucella infection in horses from an intensive system in Iran. The prevalence of brucellosis in horses was low and it had no significant impact on the transmission of infection to humans. Conclusions: Regular screening programs, along with the quarantine and elimination of animal transfer are essential for controlling the disease.