Jamal Gharekhani
Mohammad Yakhchali
Reza Khaltabadi-Farahani

Background: Neosporosis is considered as a ubiquitous disease in Iran and other countries. This research was expected to determine the prevalence and related risk factors of Neospora caninum in household dogs in Hamadan Municipality, Iran. Methods: A total of ۱۸۴ whole blood was evaluated for the presence of antibodies to N. caninum by the enzyme-linked immunosorbent assay (ELISA). All seropositive animals were affirmed by molecular techniques. Results: Based on serology and molecular methods, N. caninum infection was detected in ۴.۹% (۹۵% CI = ۴.۹ ± ۳.۱%) of animals. In addition, the highest infection rate was significantly recognized in female dogs (۵۷.۱%) with under ۶ months old (۵۴.۴%). Additionally, the clinical signs of neosporosis were observed in ۲ out of ۴ positive dogs (P<۰.۰۰۰۱, odds ratio [OR] = ۲۴.۷۱). Finally, the infection had no significant connection (P>۰.۰۵) with breeding, food regime, housing, and direct contact with infected animals. Conclusions: In general, the serological and molecular outcomes were parallel together. It was concluded that this is a universal assessment of risk factors related to N. caninum in Iranian house dogs for the first time.