Detection of Staphylococcus aureus Enterotoxin A (SEA) Using Dot-ELISA in Milk Samples

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نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JMMI-8-4_002

تاریخ نمایه سازی: 29 بهمن 1402

Abstract:

Introduction: Staphylococcus aureus enterotoxin A (SEA) is one of the most common causes of staphylococcal food poisoning. Due to the simplicity and no requirement for laboratory apparatuses, dot-ELISA is a choice method for detecting Staphylococcal enterotoxins. The present study aimed to develop a dot-ELISA for the detection of SEA. Methods: Nitrocellulose membranes were coated with the SEA antibody and blocked by the addition of ۳% bovine serum albumin (BSA) blocking buffer. After ۱ h incubation and washing the membranes, milk samples and the positive control (SEA, ۵۰ ng/ml) were added to the membranes and incubated for ۱ h. The membranes were then washed and incubated for ۴۵ min with HRP-conjugated SEA, followed by the addition of TMB. Results: Our dot-ELISA could detect amounts of ≥ ۵۰ ng/ml of SEA in the milk samples. Of the ۳۰ raw milk samples randomly purchased from dairy product stores in District ۳, Tehran, ۵ (۱۶%) contained SEA ≥ ۵۰ ng/ml by the dot-ELISA. Conclusion: The dot-ELISA showed to be a reliable method for the preliminary screening of milk samples for SEA contamination. This method is cost-effective, fast, and does not require an ELISA-reader device.

Keywords:

dot-ELISA , Staphylococcus aureus Enterotoxin A (SEA) , Raw milk

Authors

Haniyeh Golafrouz

Department of Agricultural Sciences and Food Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Hamed Ahari

Department of Agricultural Sciences and Food Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Seyed Amirali Anvar

Faculty of Veterinary Medicine, Islamic Azad University, Food Hygiene Sciences, Tehran, Iran

Delavar Shahbazzadeh

Pasteur Institute of Iran, Biotechnology Research Center, Venom and Bio therapeutic Molecules Lab., Tehran-Iran.

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