Hossein Norouzian - Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
Nader Shahrokhi - Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
Shahram Sabeti - Pathology Ward, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Saeid Bouzari - Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
Mohammad Pooya - Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran

Introduction: Antibiotic resistance, especially in Gram-negative uropathogens such as Escherichia coli, is the main barrier to treat urinary tract infection (UTI). In recent years, the dramatically increased resistance of E. coli to quinolones, a group of widely used antibiotics, has become a significant concern. Methods: In this descriptive cross-sectional study, we collected ۲۶۱ E. coli isolates from the urine and stool samples of patients, referred to or hospitalized at Loghman hospital in Tehran, Iran, with either acute or recurrent UTI. The susceptibility testing for quinolones was performed by the disk diffusion method according to the recent protocols. Results: The frequency of resistant E. coli isolates was higher against nalidixic acid than ciprofloxacin and norfloxacin (۶۷.۸% vs. ۴۸.۷% and ۴۴.۱% respectively). When comparing acute and recurrent phases of UTI, in the urine samples, no significant difference was seen in the frequency of resistant isolates against nalidixic acid and norfloxacin, while this frequency against ciprofloxacin was significantly higher in recurrent UTI (۶۸% vs. ۴۸.۲%). However, in the stool samples, the frequency of resistant isolates against nalidixic acid was higher in recurrent UTI (۷۷.۱% vs. ۵۵.۷%), while no significant difference was seen against ciprofloxacin and norfloxacin in these phases. Conclusion: Regarding the antibiotic type and frequency of the administration, the resistance pattern of E. coli to quinolones seems to differ in acute and recurrent phases of UTI.

Urinary Tract Infection, Uropathogenic Escherichia coli, Antimicrobial Susceptibility, Quinolones, Acute Disease