Ehsan Sarreshteh - Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Mosayeb Rostamian - Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Mahsa Tat Asadi - Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Firoozeh Abrishami - Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Ali Najafi - Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Maryam Abolghazi - Department of Microbiology, Qom Branch of Islamic Azad University, Qom, Iran
Hamid Mahmoudzadeh Niknam - Department of Immunology, Pasteur Institute of Iran, Tehran, Iran

Introduction: The term Leishmaniasis applies to a group of parasitic diseases caused by the genus Leishmania. Route of infection is one of the variables that have been reported to influence the immune responses as well as the disease outcome in experimental models of leishmaniasis. This research aims to study the effect of route of infection on the pathogenicity of Leishmania major in BALB/c mice. Methods: Low (۱۰۳ parasites/mouse) and high (۱۰۶ parasites/mouse) doses of L. major was injected into footpad or ear dermis of BALB/c mice. Lesion diameter was determined throughout the study. Parasite load of draining lymph nodes and spleen were assessed at three intervals. Results: Footpad in comparison to ear route showed higher pathogenicity of L. major in BALB/c mice as assessed by lesion diameter, parasite load in the draining lymph node, and dissemination of the parasite to the spleen. Conclusion: Our findings suggest that substantial differences between footpad and ear route need particular attention when we use experimental models for the study of Leishmania infections.

Leishmania major, Pathogenicity, Administration Routes, Viscera, BALB/c mice