MH Naeimi - Students research committee, Baqiyatallah University of Medical Sciences, Tehran, Iran.
MT Mohammadi - Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
M Sepandi - Department of Epidemiology and Biostatistics, School of Public Health, Baqiyatallah University of Medical Sciences, Iran.
H Ghoshooni - Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
M Rahimi Nasrabadi - Department of Chemistry, School of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran
A Gharib - Student research committee, Baqiyatallah University of Medical Sciences, Tehran, Iran
Z Bahari - Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Neuropathic pain, a chronic pain condition caused by nerve damage either of the peripheral or central nervous system, responds poorly to current drug treatments. The present study aimed to investigate the analgesic and anxiolytic effect of Fe۲+ nanoparticles on chronic constriction injury of sciatic nerve (CCI)-induced neuropathic pain in rats. We also assessed the effects of Fe۲+ nanoparticles on brain rhythmical oscillation in rats with neuropathic pain. The CCI model was induced by four loose ligations of the left sciatic nerve. Male Wistar rats were divided into four groups: control, sham, CCI, and CCI+Fe۲+ nanoparticle (۱ mg/kg). The Fe۲+ nanoparticle was administered by gavage on the day of CCI surgery (day ۰) and daily (once a day) for ۲۱ consecutive days after CCI surgery. Behavioral studies were conducted on days -۱, ۳, ۷, ۱۴, and ۲۱ after CCI. An acetone test and elevated plus maze were performed to evaluate cold allodynia and induced anxiety-like responses, respectively. A field test was conducted to evaluate innate anxiety-like behaviors. In addition, an electrophysiological study was carried out on day ۲۱ after CCI to assess the effects of drugs on brain wave power. Application of Fe۲+ significantly reduced cold allodynia in all tested days after CCI, compared to the CCI group. The obtained data demonstrated that Fe۲+ nanoparticle gavage caused analgesic and anxiolytic effects on all experimental days after CCI, compared to the CCI group. The CCI surgery significantly disturbed theta, alpha, and beta power in the brain. The application of Fe۲+ nanoparticles could not significantly change brain wave power. It is suggested that Fe۲+ nanoparticle has analgesic and anxiolytic effects during chronic neuropathic pain in rats. Furthermore, the CCI surgery effectively disturbed brain theta, alpha, and beta power. Nonetheless, the application of Fe۲+ nanoparticles could not change deregulated brain oscillation in rats.

Antioxidant, EEG, EPM, Fe۲+ nanoparticle, Neuropathic pain