Waad Almuatasem Mohieldeen - Department of Hematology and Immunohematology, Faculty of Medical Laboratory, National University, Sudan.
Albara Ahmed - Department of Hematology, Medical Laboratory Program, Alfajr College for Sciences and Technology, Sudan.
Yousif Mohammed Elmosaad - Department of Public Health, College of Applied Medical Sciences, King Faisal University, Saudi Arabia.
Rania Saad Suliman - Department of Clinical Laboratory Sciences, Prince Sultan Military College for Health Sciences, Dhahran, Saudi Arabia.
Abdulaziz Alfahed - Department of Medical Laboratory, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj ۱۱۹۴۲, Saudi Arabia.
Ahmed Hjazi - Department of Medical Laboratory, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj ۱۱۹۴۲, Saudi Arabia.
Humood Al Shmrany - Department of Medical Laboratory, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj ۱۱۹۴۲, Saudi Arabia.
Nora Hakami - Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Mohammed Ageeli Hakami - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Al- Quwayiyah, Shaqra University, Riyadh, Saudi Arabia.
Alhomidi Almotiri - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Dawadmi, Shaqra University, Riyadh, Saudi Arabia.
Hisham Ali Waggiallah - Department of Medical Laboratory, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj ۱۱۹۴۲, Saudi Arabia.

Background: A genetic polymorphism that causes abnormal folate metabolism may lead to genomic instability and increase susceptibility to malignancies such as Acute Lymphoblastic leukemia (ALL). The purpose of this research is to identify methylene tetrahydrofolate reductase (MTHFR C۶۷۷T) (NCBI ID: ۴۵۲۴) mutation in ALL patients. Methods: The study was a descriptive case-control hospital-based study with one hundred Sudanese participants divided equally into fifty (۵۰) Sudanese ALL diagnosed patients as cases and fifty (۵۰) Sudanese individuals as controls. The MTHFR C۶۷۷T mutant allele was detected using conventional PCR, with the primer sequence of MTHFR C۶۷۷T F-TGAAGGAAGGTGTCTGCGGGA R-AGGACGGTGCGGTGAGAGTG. The study was conducted from January to March ۲۰۲۳, and samples were collected from the Radiation and Isotops Center at Khartoum Hospital. Results: The investigation revealed that ۱۲ of the ۵۰ patients in the case group (۲۴%) had the MTHFR C۶۷۷T mutant allele, and the study also revealed that there is significant correlation with the control group. There is no significant relationship between socio-demographic variables and MTHFR mutation detection in ALL patients. Also, the sociodemographic variables predictors of MTHFR mutation among ALL patients adjusted for smoking habit revealed no significant relationship. Conclusion: According to the findings of this study, the mutant allele of the Methylene Tetra Hydro Folate Reductase C۶۷۷T was detected and demonstrated varying degrees of significance. It was concluded that the MTHFR C۶۷۷T gene mutation was associated with acute lymphoblastic leukemia in Sudanese patients.

ALL, MTHFR C۶۷۷T, MTHFR protein, Mutation.