Nasreen Channa - Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro۷۶۰۸۰, Pakistan
Khan Hossain - Applied Chemistry and Chemical Engineering, Rajshahi University, Bangladesh
Moina Moghal - Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro۷۶۰۸۰, Pakistan
Aamna Balouch - National Centre of Excellence in Analytical Chemistry University of Sindh, Jamshoro, ۷۶۰۸۰, Pakistan
Iqra Shahid - Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro۷۶۰۸۰, Pakistan
Umedan Channa - Institute of Biochemistry, University of Sindh, Jamshoro۷۶۰۸۰, Pakistan
Nasira Rajput - Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro۷۶۰۸۰, Pakistan
Samra Qaimkhani - Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro۷۶۰۸۰, Pakistan

In this study, formaldehyde (HCHO) and bis(۲-Hydroxy-۱-napthaldehyde)-o-phenylenediamine (BHNPhen) are heated to ۱۲۰–۱۳۰ °C for one to two hours in order to develop an effective antibacterial resin. Polymethylene bis(۲-hydroxy-۱-napthaldehyde)-o-phenylenediamine (PMBHNPhen), the resultant Schiff base, showed remarkable antibacterial qualities. Compounds consisting of a primary amine and a carbonyl molecule, which produce a chemical structure with an azomethine group (-HC=N-p), are referred to as "Schiff bases" by Hugo Schiff. The combination of Schiff bases and metal ions can lead to improvements in coordination and chemistry. The synthesized material was characterized by means of CHNS, FTIR, UV-visible spectroscopy, and SEM. analyses. The C.H.N. analytical results showed that the estimated values agreed well with the results, and the FTIR spectrum showed absorption bands at ۱۶۱۵cm-۱ and ۱۶۲۰cm-۱. A UV-visible spectroscopy investigation revealed polymerization-related changes in the absorption spectra. Significant antibacterial action was demonstrated by the synthesized Schiff base and resin against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. This work highlights the potential uses of Schiff base resin in biomedical interventions and advances the development of antibacterial materials.

polycondensation, Escherichia coli, Staphylococcus aureus, Spectroscopy