Probiotic effects of Metschnikowia isolated from dairy products aquatic environments

Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
View: 57

This Paper With 12 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_IJMCM-12-2_002

تاریخ نمایه سازی: 18 اسفند 1402

Abstract:

The aim of this study was to characterize the probiotic yeasts isolated from both aquatic environment and dairy products by sanger sequencing method and drawing phylogenetic tree for the identified probiotic yeasts. Sampling of dairy and non-dairy products was done randomly. Characterization of yeasts was constructed by molecular strategies based on the amplification and sequencing of the ribosomal DNA internal transcribed spacer region. MEGA۷ software was also applied for alignment (Muscle algorithm) and to create an agreement neighbour-joining analysis to determine the phylogenetic relationship of isolated species. For an in vitro selection of the probiotic candidates, survival of isolates at different temperatures, pH and bile salts was assessed. Based on biochemical assays and gene sequencing, the isolates were detected as Candida albicans (Wb), Clavispora lusitaniae (WC), Metschnikowia (KC) and Saccharomyces cerevisiae (Vc) strains. We indicated that Wb and KC isolates could significantly grow at ۳۷°C after ۳ hours. Wb, KC and Vc species also were proliferated at pH~۱.۵. We concluded that Wb and KC strains isolated from the marine environment and dairy products have great potential for use as probiotics in the food industry based on their resistance in human body at temperature equal to ۳۷°C and gastric PH equal to ۱.۵.

Authors

Hedieh Abolghasemi

Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran

Mohaddeseh Larypoor

Assistant professor of mycology, Department of microbiology, faculty of biological science, Islamic Azad University, Tehran North Branch, Tehran, Iran

Farzaneh Hosseini

Assistant professor of microbiology, Department of microbiology, faculty of biological science, Islamic Azad University, Tehran North Branch, Tehran, Iran