Fatemeh Abedini - Department of Laboratory Animal Breeding, Agricultural Research, Education and Extension Organization, Razi Vaccine and Serum Research Institute, PO Box ۳۱۹۷۵/۱۴۸ Hessarak, Karaj, Alborz, Iran
Mohammad Reza Zinatizadeh - MSc in Genetics, Dept. of Biology, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran
Abbas Ashtari - Mycoplasma reference laboratory, Razi vaccine and serum research institute, Karaj, Iran
mostafa jafarpour - Faculty of Biological Science, Islamic Azad University, Tonekabon Branch, Mazanderan, Tonekabon, Iran
Mohammad Ebrahimi - Quality Assurance, Razi Vaccine and Serum Research Institute, Karaj, Iran
Golamreza shokri - Research and Development Department, Agricultural Research, Education and Extension Organization, Razi Vaccine and Serum Research Institute,
Roozbeh Fallahi - Department of Research & Development, Razi Vaccine and Serum Research Institute, Karaj, Iran

Abstract Mycoplasma pulmonis and muris infections have been associated with several diseases in conventionally housed laboratory rat and mice colonies. In naturally infected of mycoplasma pulmonis in mice and rat colonies, the respiratory organ appears to be the favored site of colonization; so, it was not surprising to see that infection spread from the site of urogenital tract to the respiratory tract. In this research, PPLO broth culture and polymerase chain reaction (PCR) assay was used to detect Mycoplasma pulmonis and muris contamination in mice strain NIH. A new species of Mycoplasma muris(MYMORazi) have been identified in the nasopharyngeal, lung and vaginal samples of mice strain NIH in Razi Vaccine and serum research institute. Despite using two pair's specific primers targeting ۱۶SrRNA gene of Mycoplasma pulmonis, detection was failed in both organs. We propose that the Mycoplasma muris strain MYMO Razi can cause infection in both organs of mouse strain NIH without presence of Mycoplasma pulmonis.

Mycoplasma muris, Mycoplasma pulmonis, Laboratory Mice, Vaginal Infection, Lung Infection