Alireza Massiha - Department of Biotechnology, Lahijan branch, Islamic Azad University of Iran
Mohammad Reza Majid Khoshkholgh Pahlaviani - Department of Biotechnology, Lahijan branch, Islamic Azad University of Iran

The main purpose of the study is to determine resistance pattern of bacterial isolates to selected antibiotics among ۸۸ dyspeptic patients in Iran. Frequency of infection among these patients have been evaluated among dyspeptic patients old (۴۵ boys and ۴۳ girls, aged ۵-۱۸ years) from ۲۰۱۶ to ۲۰۱۷.Eighty-eight patients with gastroduodenal disorders were included in the study. Urease test modified Giemsa stain, culture isolation and histopathology of H.pylori isolates were performed as per standard guidelines. H.pylori ATCC ۴۳۵۰۴ was used as reference strain. Susceptibility of HP isolates to amoxicillin, tetracycline, metronidazole, clarithromycin and levofloxacin was determined using EUCAST break points by E-test method. Antral gastritis caused by H pylori infection (۱۵.۹۱%) with or without extension into the duodenum was prominent endoscopic finding. The study revealed modified Giemsa stain positivity of ۱۳.۶%, urease test positivity ۲۳.۹%, and culture positivity rate of ۱۸.۲%. The three tests Culture, Giemsa, and Urease test found to be significant statistically (P<۰.۰۵). H. pylori isolates indicated the most sensitivity against tetracycline, amoxicillin, and levofloxacin. The most rates of resistance of HP isolates were shown to Clarithromycin and metronidazole. Findings showed that the MICs of therapeutic antibiotics for eradication of HP have increased in the past ۱۰ years in northern Iran. Culture and histopathology, urease test, modified Giemsa smear and antibiogram results proved to be helpful in the diagnosis of H. pylori infection. Antimicrobial susceptibility testing can play an important role in experimental treatment of HP infection and management of drug resistant cases of isolates in our country.

Gastrointestinal disorders, Helicobacter pylori, Antibiotic resistant, E-test, Iran