Azam Ghorbani - Assistant Professor of Islamic Azad University of Saveh Department of Chemistry, Collage of Technology and Engineering, Saveh Branch,Islamic Azad University,Saveh, Iran

In This study three-phase hollow fibers liquid-phase micro extraction (LPME) method for preconcentration of Cefixime from biological sample was used. Cefixime is an antibiotic medication used to treat a number of bacterial infections. The extracted analyte with LPME was detected with high performance liquid chromatography equipped with ultraviolet detector (HPLC-UV). The effect of various parameters which affected extraction efficiency such as type of organic solvent, receiving phase, pH of receiving phase and extraction phase, extraction time and mixing speed were investigated. Under optimum condition of extraction condition the pre-concentration factor was obtained ۶۱۸ for urine samples with an appropriate detection limit of ۲ µg ml-۱. The real sample study is provided satisfactory extraction recovery for urine (۷۴%) samples.

- Hollow fiber liquid phase micro extraction; biological sample; Cefixime; High performance liquid chromatography