Saeed Heydaryan - Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Dariush Shirani - Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Arash Ghalyanchi Langeroudi - Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Saied Bokaie - Department of Epidemiology, Faculty of Veterinary Medicine, University of Tehran. Tehran, Iran.
Mehdi Hassankhani - Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Ali Roustaei - Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Leyili Halimiasl - Department of Veterinary Medicine, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Background: In cats, hypertrophic cardiomyopathy (HCM) stands out as a prevailing heart disease. The mutations in the gene that encodes cardiac myosin-binding protein C (MYBPC۳) have been detected in the Ragdoll and Maine Coon breeds. Objectives: HCM is believed to be hereditary in other breeds, too. Methods: Blood samples were collected for DNA extraction from ۲ unaffected and ۷ affected Persian breed cats with HCM. Besides accomplishing conventional polymerase chain reaction, DNA sequencing was performed. The sequence changes were utilized to detect single nucleotide polymorphisms in the MYBPC۳ gene and predict amino acid substitutions based on the Acc. No. XM_۰۱۹۸۱۲۳۹۶.۱ and comparisons with the literature on identified breed variants and control samples.Results: Although many single nucleotide polymorphisms were found in the affected and unaffected Persian cats, no causative mutation for HCM was observed.Conclusion: In this breed, HCM does not seem to be caused solely by mutations in this cardiac gene. Potential cardiac genes should be investigated to uncover other genetic reasons for this cardiac disease in the Persian cat breed.

Hypertrophic cardiomyopathy (HCM), Myosin-binding protein C۳, Persian Breed, Cat, Polymerase Chain Reaction (PCR)