Prediction of the best signal peptide for periplasmic expression of melittin peptide in Escherichia coli

Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JPSAD-2-2_006

تاریخ نمایه سازی: 3 اردیبهشت 1403

Abstract:

Periplasmic expression of antimicrobial peptides is one of the most important issues in cloning and protein expression systems. In the present study, bioinformatics methods were used to predict the best signal peptides for periplasmic expression of the melittin peptide in Escherichia coli. Therefore, the sequence of ۱۲ signal peptides was prepared from signal peptide databases. In order to choose the best signal peptide, periplasmic expression, sub-cellular localization site, solubility, physical, and chemical properties of signal peptides were analyzed by Signalp۶, Psort, Protein-sol, PRED-TAT, and Portparam servers. Out of ۱۲ signal peptides, six passed the Signalp۶ filter, and only two passed Psort. After examining the solubility of signal peptides and investigating their physical and chemical properties, it was determined that zraP has the most favorable characteristics. Finally, it can be concluded that zraP could be considered as the best signal peptide for the melittin expression. Our results can be used for periplasmic expression of melittin in Escherichia coli host.Periplasmic expression of antimicrobial peptides is one of the most important issues in cloning and protein expression systems. In the present study, bioinformatics methods were used to predict the best signal peptides for periplasmic expression of the melittin peptide in Escherichia coli. Therefore, the sequence of ۱۲ signal peptides was prepared from signal peptide databases. In order to choose the best signal peptide, periplasmic expression, sub-cellular localization site, solubility, physical, and chemical properties of signal peptides were analyzed by Signalp۶, Psort, Protein-sol, PRED-TAT, and Portparam servers. Out of ۱۲ signal peptides, six passed the Signalp۶ filter, and only two passed Psort. After examining the solubility of signal peptides and investigating their physical and chemical properties, it was determined that zraP has the most favorable characteristics. Finally, it can be concluded that zraP could be considered as the best signal peptide for the melittin expression. Our results can be used for periplasmic expression of melittin in Escherichia coli host.

Authors

Dr. Amir Asghari Baghkheirati

۰۹۳۵۶۰۳۴۵۱۹

Elnaz Karbaschian

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Seyedah Zahra Mousavi

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Mohammad Hadi Sekhavati

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.