Mohadeseh Mirnikjoo - Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Annahita Rezaie - Department of pathobiology, Faculty of Veterinary medicine, Shahid Chamran University of Ahvaz
Mohammad Mehdi Dehghan - Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Hadi Naddaf - Department of Clinical sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Objective: In this study, the bone regeneration ability of polyacrylamide hydrogel and nanohydroxyapatite scaffolds (PAAH/NHA) and stem cells derived from adipose tissue (ADSCs) in the healing of critical sized bone defects in rabbit radius were assessed. Animals and procedures: ۱۲ New Zealand white male rabbits were divided into ۳ groups. The rabbits were anesthetized and ۱۵ mm bone defects were created on the radius. According to the group, scaffold with or without ADSCs were implanted into the defects. These groups were as follows: A (control group, bone defect without any treatment), B (embedding of PAAH/NHA scaffolds in the bone defect) and C (embedding of PAAH/NHA scaffolds with stem cells derived from adipose tissue in the bone defect). The animals were euthanized ۱۲ weeks after surgery and histopathologic evaluation were carried out.Result and conclusion: microscopic examination revealed significant increase in new bone formation in group C than B. The results of this study showed that the use of stem cells derived from adipose tissue implanted on PAAH/NHA scaffold can stimulate osteogenesis.

Adipose derived stromal cells, polyacrylamide hydrogel, nanohydroxyapatite, Bone