Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs

Objective- To evaluate the effects of autologous platelet-rich plasma (PRP) on wound healing in dogs. Design- Experimental in-vivo study. Animals- ۵ mix-breed male adult dogs. Procedures- Under general anesthesia, six ۲ × ۲-cm, full-thickness skin wounds were created on the back of dogs symmetrically. In each dog, three right side wounds were treated topically with ۱.۵ ml PRP jelly, whereas left side wounds received no treatment. For macroscopic evaluation, at days ۰, ۳, ۵, ۷, ۱۰, ۱۳, ۱۷, ۲۰, and ۲۴, digital photographs were taken from wounds. At days ۱۰, ۱۷ and ۲۴ after wounding, skin biopsies were taken from the center and corner of each wound for hydroxyprolin measurement and histopathologic evaluation respectively. Results- No statistically significant differences were found in percentage of wound contraction, epithelialization and healing between test and control group during study (P>۰.۰۵). There were no significant differences between median of hydroxyprolin levels, median of inflammatory cells infiltration, presence of dermal granulation tissue, fibroblast proliferation, arrangement of fibroblasts, collagen deposition and collagen bundle formation scores, in the specimens from left and right wounds (P>۰.۰۵). Conclusion and Clinical Relevance- Topical application of platelet-rich plasma can not accelerate repair of the small granulating wounds of dogs.

Keywords:

Dog , wound healing , Platelet- rich plasma , Growth Factors