Genetic relationships among collections of the Persian sturgeon, Acipenser percicus, in the south Caspian Sea detected by mitochondrial DNA Restriction fragment length polymorphisms

Publish Year: 1391
نوع سند: مقاله ژورنالی
زبان: English
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JR_CJES-10-2_013

تاریخ نمایه سازی: 21 خرداد 1403

Abstract:

In the present study, mitochondrial DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to assess the population structure and genetic relationships among six Persian sturgeon, Acipenser persicus populations from south Caspian Sea along the Iranian coast. The complete nucleotide dehydrogenase subunit ۵ (NADH ۵) region of mtDNA amplified by PCR was digested with five restriction enzymes. In total, ۱۵۴ individuals from six populations including: Guilan (Zone۱-۲), Mazandaran (Zone ۳ and ۵), Golestan (Zone ۴) and Sefidroud River, from south Caspian Sea along the Iranian coast were analyzed using five restriction endonucleases (Rsa ?, Hinf ?, HaeIII, Mbo ? and Cfr۱۳?), yielding ۱۷ haplotypes. Samples from Sefidroud River were clearly identified by cluster and molecular variance model (AMOVA) analyses. This collection showed dominant haplotypes that were little in populations from the other geographic areas. The mean haplotype diversity (h) and nucleotide diversity (?) were ۰.۷۳۹?۰.۰۳۸ and ۰.۰۱۰۵?۰.۰۰۴۳, respectively. Based on heterogeneity test haplotype frequencies of Persian sturgeon populations and Monte-Carlo with ۱۰۰۰ replicates in PCR-RFLP method significant differences were seen (?۲ =۳۷.۱۲, P< ۰.۰۰۰۱) and these results showed that haplotype distribution in different location were significant and populations of Sefidroud were statistically significant (P< ۰.۰۰۰۱). This result suggests that the unique genetic structure of Sefidroud River represents a highly valuable genetic resource and should now be treated as demographically independent and managed separately.

Authors

M. Pourkazemi

M. Khoshkholgh۱ , M. Pourkazemi۲, S. Nazari۱, L. Azizzadeh Pormehr۲

al. et

O. Rafieyan ۱, A. A. Darvishsefat۲, S. Babaii۱, A. Mataji۱

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  • Ataei, M., Pourkazemi, M., Hasanzadeh Kiabi, B and Shedaei, M. ...
  • Billington, N. (۲۰۰۳). Mitochondrial DNA. Pages ۵۹–۱۰۰ in E. M. ...
  • Birstein V.I, Bemis W.E, and Waldman J.R. (۱۹۹۷). The threatened ...
  • Brown K.H. (۲۰۰۸). Fish mitochondrial genomics: sequence, inheritance and functional ...
  • Caughley G. & Gunn A. (۱۹۹۶) Conservation Biology in Theory ...
  • Excoffier L., Laval G., and Schneider S. (۲۰۰۵). Arlequin Ver. ...
  • Genetic relationships of the Persian sturgeon۲۲۴Felsenstein, J., (۱۹۸۵). Confidence Limits ...
  • Felsenstein J. (۱۹۹۵). PHYLIP, Phylogeny inference package, version ۳.۵۷c. Department ...
  • Hillis, D and Moritz, M. C. (۱۹۹۰). Molecular taxonomic. Sinauer ...
  • Khoshkholgh, M.(۲۰۰۷). Comparision on genetic structure of Persian sturgeon (Acipenser ...
  • Ludwig A, Kirschbaum F (۱۹۹۸) Comparison of mitochondrial DNA sequences ...
  • McElroy, D.M., Moran, P., Bermingham, E., and Kornfield, I., (۱۹۹۲). ...
  • McNeely J.A., Miller K.R., Reid W.V., Mittermeier R.A. & Werner ...
  • Moghim M., Kor D., Tavakolieshkalak M. & Khoshghalb M.B. (۲۰۰۶). ...
  • Moritz C. (۱۹۹۴). Applications of mitochondrial DNA analysis in conservation: ...
  • Nei, M., and Tajima, F., (۱۹۸۱). DNA polymorphism detectable by ...
  • Lalouie, F., (۱۹۹۶) Study on Nature Reproduction in sturgeon of ...
  • Pikitch E.K., Doukakis P., Lauck L., Chakrabarty P. & Erickson ...
  • Pourkazemi M. (۱۹۹۶). Molecular and biochemical genetic analysis of sturgeon ...
  • Pourkazemi M., Skibinski, D.O.F. & Beardmore J.A., (۱۹۹۹). Application of ...
  • Pourkazemi, M. (۲۰۰۶). Caspian Sea sturgeon Conservation and Fisheries: Past ...
  • Pourkazemi, M. (۲۰۰۸). Comprehensive project of genetic stock assessment in ...
  • Putilina L.A. and E.N. Artyukhin. (۱۹۸۵). Some features of Persian ...
  • Rezvani Gilkolaie S. (۲۰۰۰). Study of mtDNA variation of Russian ...
  • Roff, D. and Bentzen, P., (۱۹۸۹). The Statistical Analysis of ...
  • Schneider, S., D. Roessli, and L. Excoffier. (۲۰۰۰). Arlequin: a ...
  • Shabani A., Pourkazemi M., Kamali A., Rezvani Gilkolaie S. & ...
  • Swofford, D.L., (۲۰۰۲). PAUP: Phylogenetic Analysis Using Parsimony (and Other ...
  • Weir B.S.& Cockerham C.C. (۱۹۸۴). Estimating F-statistics for the analysis ...
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