Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea

Publish Year: 1390
نوع سند: مقاله ژورنالی
زبان: English
View: 25

This Paper With 8 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_CJES-9-2_012

تاریخ نمایه سازی: 21 خرداد 1403

Abstract:

In order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from ۵ male and ۵ female fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic DNA (PCR-RAPD) was performed using ۱۲۴ primer sets. All bands were numbered using ۱ and ۰ scores corresponding to the presence or absence of bands, respectively and data were analyzed using RAPDPLOT program. Results indicated that ۴۴ sets of primers did not show any flanking site and produced no bands, while the remaining ۸۰ produced sharp and visible bands on polyacrylamid gel. In total, ۱۶۰۰ bands were scored. However, none of the bands corresponded to either the male or female fish. According to the results it has been concluded that RAPD technique failed to detect sex and cannot be considered as a robust molecular tool for sex differentiation in the studied fish. The reason may be the absence of sex chromosomes in this species or that the genes corresponding to sex differentiation are spread on different autosomal chromosomes with interaction of some environmental factors.   REFERENCES  Abdolmaleki, Sh. and Ghani Nejad, D. (۲۰۰۷). Releasing Mahi Sefied Fry and Its Role in Reconstructing the Stock of this Fish in the Iranian Coasts of the Caspian Sea, Monthly Magazine of Aquatics, ۸th year, pp. ۸-۱۴ (In Persian). Allendorf, F.W ; Gellman, W.A. and Thorgaard, G.H. , ۱۹۹۴. Sex-linkage of two enzyme loci Oncorhynchus mykiss (rainbow trout). Heredity ۷۲: ۴۹۸- ۵۰۷. Azari Takami, Gh. (۱۹۸۴). Principles of Fish Breeding and Culture, Organization of Aquatics Breeding and Development, Deputy Aquatic Fisheries Publications, p. ۱۵۲ (In Persian). Bardakci, F., ۲۰۰۰. The use of random amplified polymorphic DNA (RAPD) markers in sex discrimination in Nile Tilapia, Oreochromis niloticus (Pisces: Cichlidae), Turk J Biol, pp. ۱۶۹-۱۷۵. Baroiller J. F. and D'cotta, H., ۲۰۰۱. Environment and sex determination in farmed fish. Comp Biochem Physiol C Toxicol Pharmacol ۱۳۰: ۳۳۹-۴۰۹. Callejas, C. and Ochando, M.D., ۲۰۰۲. Phylogenetic relationships among Spanish Barbus Species(Pisces, Cyprinidae) shown by RAPD markers. Heredity ۸۹: ۳۶-۴۳. Callejas. C. and Ochando, M.D., ۲۰۰۱. Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the lberian peninsula. Jornal of Fish Biology. Volume ۵۹: ۱۵۸۹-۱۵۹۹. Congiu, L.; Rossi, R. and Colom. G., ۲۰۰۲. Population analysis of the sand smelt Atherina boyeri (teleostei. Atherinidae), from Italian coastal lagoons by random amplified polymorphic DNA. Marine Ecology, ۱, ۲۲۹: ۲۷۹-۲۸۹. Cui, J. Z.; Shen, X. Y.; Gong, Q. L.; Yang, G.P. and Gu, Q. Q., ۲۰۰۶. Identification of Sex markers by cDNA-AFLP in (Takifugu rubripes). Aquaculture, ۲۵: ۳۰-۳۶. Devlin, R. H. and Nagahama, Y., ۲۰۰۲. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture ۲۰۸, pp. ۱۹۱- ۳۶۴. Ellegren, H., ۲۰۰۱. Hens, cocks and avian sex determination. A quest for genes on Zor W? Embo reports, ۲: ۱۹۲- ۱۹۶. Fernando, A. A. and Phang, V. P. E., ۱۹۸۹. Inheritance of the tuxedo and blond tuxedo color pattern phenotypes of the guppy, Poecilia reticulate. Proceeding of the Second Asian Fisheries Forum, Tokyo, Japan, the Second Asian Fisheries Forum, Tokyo, Japan, pp. ۴۸۷- ۴۹۰. Froschauer, A., Kortin, C., Aoki, T. and Asakawa, S. , ۲۰۰۵. Construction and initial analysis of bacteria artificial chromosome (BAC) Cotigs from the sex determination region of the platyfish (Xiphophoorus maculatus). Gene, ۲۹۵: ۲۴۷-۲۵۴. Griffiths, R.; Orr, K.J., Adam, A. and Barber, I., ۲۰۰۰. DNA sex identification in the theree spined stickleback. J. Fish Biol., ۵۷: ۱۳۳۱- ۱۳۳۴. Horng, Y.M.; Wu, C., Wang, Y.C. and Huang, MC, ۲۰۰۶. A novel molecular genetic marker for gender determination of pigeons, Theriogenology ۶۵: ۱۷۵۹ - ۱۷۶۸. Itrurra, P., Medrano, J.F., Bagley, M., Lam, N., Vergara, N. and Marin, JC. , ۱۹۹۸. Identification of sex chromosome molecular, marker using RAPDs and fluorescent in situ hybridization in rainbow trout. Genetica, ۱۰۱: ۲۰۹- ۲۱۳. Iturra, P., Lam, N., Fuente, M., Vergara, N. and Medrano, J.F., ۲۰۰۱. Characterization of sex chromosomes in rainbow trout and coho salmon using Fluorescent In Situ Hybridization (FISH). Genetica ۱۱۱, pp. ۱۲۵ -۱۳۱. Jaillon, O., Aury, J.M., Petit, J. and Stange-thomann, N., ۲۰۰۴. Genome duplication in the teloset fish Tetradon nigroviridis reveals the early vertebrate proto- karyotype. Nature, ۴۳۱: ۹۴۶ -۹۵۷. Jian zhou, C.; Xue, Y. S.; Qing, L.G.; Guan Ping, and Y. Qin. Qun, G., ۲۰۰۶. Identification of sex markers by cDNA-AFLP in (Takifugu rubripes). Aquaculture, ۲۵۷: ۳۰-۳۶. Keyvanshoukouh, S; Pourkazemi M. and Kalbasi M.R. (۲۰۰۴). Investigating the Possibility of Determining the Sex of Huso huso Using the PCR-RAPD Method, Iranian Fisheries Scientific Magazine, No.۱, ۱۳th year, Spring ۲۰۰۴, pp. ۱۴۹-۱۶۲ (In Persian). Kovacs, B., Egedi, S.; Bartfai, R. and Orban, L., ۲۰۰۱. Male-specific DNA markers from African catfish (Clarias gariepinus). Genetica, ۱۱۰: ۲۶۷-۲۷۶. Levin, I.; Crittenden, L.B. and Dodgson, J.B., ۱۹۹۳. Genetic map of the chicken. Z chromosome using random amplification polymorphic DNA. Genomics ۱۶: ۲۲۴-۲۳۰. Li, Y. ; Hill, J.A. ; Yue, G.H. ; Chen, F. and Orban, L., ۲۰۰۲. Extensive search does not identify genomic sex markers in Tetradon nigroviridis. Journal of Fish Biology. ۶۱: ۱۳۱۴-۱۳۱۷. MaGowan, C. and Davidson, W.S., ۱۹۹۸. The RAPD technique fails to detect a male-specific genetic marker in Atlantic salmon. Journal of Fish Biology, ۵۳:  ۱۱۳۴- ۱۱۳۶. Martinez, E. A.; Destombe, C.; Quillet, M. C. and Valero, M., ۱۹۹۹. Identification of random amplified polymorphic DNA (RAPD) markers highly linked to sex determination in the red alga Gracilaria gracilis. Molecular Ecology, ۸: ۱۵۳۳-۱۵۳۸. Matsuda, M.; Matsuda, C.; Hamaguchi, S. and Sakaizumi, M., ۱۹۹۸. Identification of the sex chromosomes of the medaka, Oryzias latipes, by fluorescence in situ hybridization. Cytogenet. Cell Genet. ۸۲: ۲۵۷- ۲۶۲. McQuown, E.; Sloss, B. L.; Sheehan, R. J. and May, B., ۲۰۰۰. Microsatellite analysis of genetic variation in Sturgeon (Acipenseridae): new primer sequence for Scaphirhychus and Acipenser. Trans. Am. Fish. Soc. ۱۲۹: ۱۳۸۰-۱۳۸۸. Penalva, L. and Sanchez, L., ۲۰۰۳. RNA binding protein sex- lethal (sxl) and control of Drosophila sex determination and dosage compensation. Microbial Mol. Biol Rev, ۶۷, pp. ۳۴۳-۳۵۹. Pourkazemi, M. , ۱۹۹۶. Molecular and biochemical genetic analysis of sturgeon stocks from the South Caspian Sea. PhD Dissertation, University of Wales, Swansea. ۲۶۰ p. Ravelo, C.; Magarinos, S. and Toranzo, A.E., ۲۰۰۲. Molecular fingerprinting of fish pathogenic Lactoccus garvieae strains by random amplified polymorphic DNA analysis. Jornal of Clinical Microbiology, pp. ۷۵۱-۷۵۶. Razavi Sayyad B.A. (۱۹۹۰). Evaluation and Management of Economic Fish Stock of the Caspian Sea, Fishery Research organization, Guilan Province, Bandar Anzali, p. ۸۶ (In Persian). Roest Crollius, H. and Weissebach, J., ۲۰۰۵. Fish genomic and biology. Genom Res ۱۵, pp. ۱۶۷۵-۱۶۸۲. Schartl, M., ۲۰۰۴. Sex chromosome evolution in non mammalian vertebrates. Curr Opin Genet Dev. ۱۴: ۶۳۴-۶۴۱. Sun, X. Liang, L., ۲۰۰۳. A genetic linkage map of common carp (Cyprinus carpio L.) and mapping of a locus associated with cold tolerance. Aquaculture, ۲۳۸: ۱۶۵-۱۷۲.  Tave, D. ۱۹۹۳. Genetics for fish hatchery managers ۲nd edn. Van Nostrand Reinhold, New York, ۴۱۵ p. Wardell, B.; Sudweeks, J. D.; Meeker, N. D.; Estes, S. S.; Woodward, S.R.; and Teuscher, C., ۱۹۹۳. The identification of Y chromosome-linked markers with arbitrary primers. Nucleic Acids Reserch, ۱۸: ۷۲۱۳-۷۲۱۸.  Welsh, J. and McClelland, M., (۱۹۹۰). Fingerinting genomes using PCR with arbitrary primers. Nucleic Acids Research, ۱۸: ۷۲۱۳-۷۲۱۸. Williams, J. G. K., Kabelik, A.R., Liva, K. J., Rafalski, J.A., and Tingey, V. , (۱۹۹۰). DNA polymorphism Amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research, ۱۸: ۶۵۳۱-۶۵۳۵. Würtz, S, Gaillard, S, Barbisan, F, Carle, S, Congiu, L., Forlani, A, Aubert, J, Kirschbaum, F, Tosi, E, Zane, L. and Grillasca, J (۲۰۰۶). Extensive screening of sturgeon genomes by random screening techniques revealed no sex-specific marker. Aquaculture, ۲۵۸: ۶۸۵-۶۸۸.

Keywords:

Mahisefid , Rutilus frisii kutum , Random Amplified Polymorphic DNA , Sex determination

Authors

M. Pourkazemi

Sturgeon International Research Institute, Rasht, Iran, P.O.Box: ۴۱۶۳۵-۳۴۶۴.

S. Razikazemi

Payam-e-Noor University, School of Science, Tehran Branch, P.O. Box: ۱۹۲۹۵-۴۶۹۷ Corresponding author’s E-mail: pkazemi_m@yahoo.com

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Abdolmaleki, Sh. and Ghani Nejad, D. (۲۰۰۷). Releasing Mahi Sefied ...
  • Allendorf, F.W ; Gellman, W.A. and Thorgaard, G.H. , ۱۹۹۴. ...
  • Azari Takami, Gh. (۱۹۸۴). Principles of Fish Breeding and Culture, ...
  • Bardakci, F., ۲۰۰۰. The use of random amplified polymorphic DNA ...
  • Baroiller J. F. and D'cotta, H., ۲۰۰۱. Environment and sex ...
  • Callejas, C. and Ochando, M.D. , ۲۰۰۲. Phylogenetic relationships among ...
  • Callejas. C. and Ochando, M.D. , ۲۰۰۱. Molecular identification (RAPD) ...
  • Congiu, L.; Rossi, R. and Colom.G. , ۲۰۰۲. Population analysis ...
  • Cui, J. Z.; Shen, X. Y.; Gong, Q. L.; Yang, ...
  • Devlin, R. H. and Nagahama, Y., ۲۰۰۲. Sex determination and ...
  • Ellegren, H., ۲۰۰۱. Hens, cocks and avian sex determination. A ...
  • Fernando, A. A. and Phang, V. P. E., ۱۹۸۹. Inheritance ...
  • Froschauer, A. ; Kortin, C. ; Aoki, T. and Asakawa, ...
  • Griffiths, R. ; Orr, K.J. ; Adam, A. and Barber, ...
  • Horng, Y.M. ; Wu, C. ; Wang, Y.C. and Huang, ...
  • Pourkazemi and Razikazemi ۲۴۱Itrurra, P. ; Medrano, J.F. ; Bagley, ...
  • Iturra, P. ; Lam, N. ; Fuente, M. ; Vergara, ...
  • Jaillon, O. ; Aury, J.M. ; Petit, J. and Stange-thomann, ...
  • Jian zhou, C.; Xue, Y. S.; Qing, L.G.; Guan Ping, ...
  • Keyvanshoukouh, S; Pourkazemi M. and Kalbasi M.R. (۲۰۰۴). Investigating the ...
  • Kovacs, B.; Egedi, S.; Bartfai, R. and Orban, L., ۲۰۰۱. ...
  • Levin, I. ; Crittenden, L.B. and Dodgson, J.B. , ۱۹۹۳. ...
  • Li, Y. ; Hill, J.A. ; Yue, G.H. ; Chen, ...
  • MaGowan, C. and Davidson, W.S. , ۱۹۹۸. The RAPD technique ...
  • Martinez, E. A.; Destombe, C.; Quillet, M. C. and Valero, ...
  • Matsuda, M.; Matsuda, C.; Hamaguchi, S. and Sakaizumi, M., ۱۹۹۸. ...
  • McQuown, E.; Sloss, B. L.; Sheehan, R. J. and May, ...
  • Penalva, L. and Sanchez, L. , ۲۰۰۳. RNA binding protein ...
  • Pourkazemi, M. , ۱۹۹۶. Molecular and biochemical genetic analysis of ...
  • Ravelo, C. ; Magarinos, S. and Toranzo, A.E., ۲۰۰۲. Molecular ...
  • Razavi Sayyad B.A. (۱۹۹۰). Evaluation and Management of Economic Fish ...
  • Roest Crollius, H. and Weissebach, J. , ۲۰۰۵. Fish genomic ...
  • Sun, X. Liang, L. , ۲۰۰۳. A genetic linkage map ...
  • Tave, D. ۱۹۹۳. Genetics for fish hatchery managers ۲nd ed. ...
  • Wardell, B.; Sudweeks, J. D.; Meeker, N. D.; Estes, S. ...
  • Welsh, J. and McClelland, M., (۱۹۹۰). Fingerinting genomes using PCR ...
  • Williams, J. G. K.; Kabelik, A.R. ; Liva, K. J. ...
  • Würtz, S. ; Gaillard, S. ; Barbisan, F. ; Carle, ...
  • نمایش کامل مراجع