Karyology study on Bleak (Alburnus alburnus) from the South Caspian Sea region

The chromosomal spread and karyotype of Bleak (Alburnus alburnus) from Anzali lagoon were identified using tissue squashing techniques with injection of ۰.۵ml/۱۰۰g body weight of ۰.۰۱% Colchicines solution in fish fingerlings. Kidney and gill tissues were then extracted and chopped in KCl ۰.۰۴۵M for ۲۰ min and fixed in Carnoy solution in ۳ stages. The chromosomal spreads were stained in ۲۰% Gimsa for ۳۰ min. From ۳۴۷ chromosomal spread counts, the results showed diploid chromosome number of this species ۲n=۵۰. Karyotype composed of ۷ metacentric, ۱۳ submetacentric and ۵ acrocentric or subtelocentric choromosome pairs, and the number of chromosome arms (NF) was determined as NF=۹۰.   REFERENCES Abdouli, A. (۱۹۹۹) The inland water fishes of Iran. Natural and wild life museum of Iran, Tehran, ۳۷۸ P. Al-Sabti, K. (۱۹۹۱) Hand book of genotoxic effects and fish chromosomes. Ljubljana, ۹۷P. AI-Sabti, K. 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Keywords:

Alburnus alburnus , Anzali Lagoon , Bleak , Chromosome , Iran , Karyotype