Investigating the effect of herbal extracts of oak, tea and chamomile on Aspergillus sp. in cultured shrimps in laboratory conditions
Publish place: 6th International Conference on Biology and Earth Sciences
Publish Year: 1402
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
BIOLOGY06_168
تاریخ نمایه سازی: 22 خرداد 1403
Abstract:
Introduction: Today, proliferation and breeding of aquatics, particularly shrimp, is of great importance due to the presence of proteins and mineral rich materials as well as short duration of its proliferation and breeding. Unfortunately, in some conditions due to the presence of some fungi such as Aspergillus, irreparable losses are imposed to shrimp farming industry. Today, the desire to use medicinal herb to improve diseases led this study to investigate the effect of three medicinal herb extracts including tan bark, green tea and chamomile on pathogenesis of Aspergillus in shrimp. Methods: The Aspergillus samples were prepared from Scientific and Industrial Research Organization of Iran and Pasteur Institute of Iran. They were maintained in Potato Dextrose Agar (PDA) gradient culture for ۵-۷ days at ۲۵ ˚C. Mycelium causes disruption in the culture and reduces the impact of the extracts. To remove them from fungal suspension, the suspension was filtered using glass wool. Then, light transmittance from the suspension was measured using a spectrophotometer device at a wavelength of ۵۳۰ nm. The light transmittance of ۹۰% is required to obtain a suspension with approximately ۱۰۶ fungal spores per ml. Five test tubes including yeast extract (YES) were prepared for each extract and totally ۱۵ medium cultures were obtained. The extracts with concentrations of ۲۰۰, ۴۰۰, ۶۰۰, ۸۰۰, ۱۰۰۰ ppm with Tween were added to yeast extract (YES) medium. The tubes were vortexed to maintain the suspension and the extracts for ۶۰ seconds and they were visually assessed up to ۴ days in terms of degree of growth or lack of growth. The contents of the tubes were then filtered using Whatman Grade ۴۲ filter paper and mycelial dry weight was obtained by a special scale. Results: Among ۳ studied extracts, chamomile extract had the best inhibitory effect on Aspergillus and tea extract had the weakest inhibitory effect. The inhibitory effect of tan bark extract was near to the effect of chamomile extract so that they both had approximately similar effect on fungal growth at concentrations of ۶۰۰ and ۱۰۰۰ ppm. The impact of tan bark extract at the concentration of ۲۰۰ ppm on inhabitation of fungal growth had better, with a little difference, from chamomile extract. Discussion and Conclusion: Due to the proper effects of chamomile extract at concentrations of ۸۰۰ and ۱۰۰۰ ppm and tan bark extract at concentrations of ۶۰۰ and ۱۰۰۰ ppm, which both showed the best and proper in vitro inhibitory effect on Aspergillus and on mycelial dry weight, their usage is recommended for treatment and prevention of aspergillosis.
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Authors
Ali Sadeghi Limanjoob
۱۰th grade student of experimental science field of Saadat government model high school, Kazeroon city, Fars, Iran.
Nahid Siahmard
PhD in Physiology and Research Director of Shahid Soleimani Student Center, Kohchanar, Fars, Iran.