Moldir Azhimakhan - Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan
Bibigul Beisembina - Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan
Anastasiya Kapytina - Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
Nazym Kerimbek - Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
Dilyara Gritsenko - Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
Dianqiu Lv - Southwest University, Chongqing, China
Hu Baigeng - Leling Xisen Potato Industry Group Company Ltd, Leling, China
Vadim Khassanov - Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan

This research studied ten isolates of potato virus X (PVX) isolated from potato cultivars growing in Kazakhstan (KZ), Belarus (BY) and China (CN). Additionally, the potato samples were screened for Potato virus Y (PVY), Potato virus S (PVS), and Potato leafroll virus (PLRV) using multiplex reverse transcription-polymerase chain reaction (RT-PCR). PVX was isolated from the potato varieties ‘Tamasha’, ‘Red Scarlet’, ‘Gala variety’, ‘Krone’, ‘Juwel’, ‘Gala’, and ‘b۲-۷’. The identified isolates of PVX were sequenced and used to construct a phylogenetic tree, which demonstrated that KZ and BY isolates form clusters closely related to isolates from Asia and Europe, however, CN isolate formed a separate clade. KZ۵ and KZ۷ PVX isolates showed local symptoms of mosaic and light green speckled spots on the upper leaves of Datura stramonium in pathogenicity test.

Potato, Potato Virus X, isolates, ELISA, PCR, Targeted sequencing, Indicator plants