Effect of Sterculia quadrifida R. Br Stem Bark on Hematological Profile and Thrombopoietin Levels in DENV-۳-infected Wistar Rats

Dengue is an infectious disease that induces various alterations in the hematological profile and THPO levels that correlate to the disease severity. This study aims to investigate the effect of Sterculia quadrifida R. Br stem bark extract on the hematological parameters and THPO levels of DENV-۳-infected Wistar rats. A total of ۲۷ male rats were divided into three groups: healthy, dengue, and treatment (dengue and extract). The extract was orally delivered at a ۱۵۰۰ mg/KgBW dose once daily for seven days. Blood samples were collected on days ۱ (healthy group), ۵, and ۸ (dengue and treatment group). The platelet, MCV, MCH, and MCHC in the dengue and treatment group showed no significant difference (p>۰.۰۵) on days ۵ and ۸ compared to the normal values, ۸۷۶ ± ۴۴.۱۱ ۱۰۳/mm۳, ۵۶.۳۴ ± ۱.۴۲ fL, ۱۸.۵۶ ± ۰.۵۴ pg, and ۳۲.۸۸ ± ۰.۵۶ g/dL, respectively. Compared to the healthy group, hematocrit, leukocyte, hemoglobin, and erythrocyte in the dengue and treatment group displayed significant differences on both days and RDW-CV on day eight alone. Post-hoc test on the fifth day revealed that the treatment group showed significant differences in hematocrit and leukocyte levels compared to the dengue group. THPO levels on day five in the dengue and treatment group were displayed at ۳۰.۲۸ ± ۶.۷۲ pg/ml and ۳۵.۷۵ ± ۲۸.۵۵ pg/ml, respectively, showing statistical difference (p<۰.۰۵) compared to the healthy group, ۴۰.۳۲ ± ۷.۷۲ pg/ml. The results showed that S. quadrifida can reduce leukocytosis and hemoconcentration and might also have the potential to increase THPO levels during dengue infection. This study was the first to report dengue-infected Wistar rats' hematological profiles and THPO levels.

Keywords:

dengue , hematological profiles , Sterculia quadrifida R. Br , thrombopoietin , Wistar rats