Pro-Oxidant/ Antioxidant Balance Correlates with Red Blood Cell Indices and Anemia Severity in the Anemic Patients

Background: The oxidant/ antioxidant balance is disrupted in anemia. Antioxidant capacity depends on antioxidant enzyme activity and some trace elements. This study aimed to evaluate oxidant/ antioxidant status and its correlation with red blood cell indices and anemia severity in anemic patients. Methods: Blood samples were taken from ۹۰ anemic patients and ۹۵ healthy people. Circulatory miR-۱۲۲ was assayed by real-time PCR. Malondialdehyde (MDA), pro-oxidant/ antioxidant balance (PAB), supper oxide demitasse (SOD), glutathione peroxidase (GPxs) activity, total antioxidant capacity (TAC), and zinc were measured by colorimetric method. Selenium was also determined using atomic absorption. Results: Selenium and zinc decreased significantly in the case group (**P=۰.۰۰۴ and ***P=۰.۰۰۰). The amount of miR-۱۲۲ up-regulated in the anemia (**P=۰.۰۰۳). MDA was significantly raised in the case vs control (***P=۰.۰۰۰۲). PAB was higher in the case group (**P=۰.۰۰۵). SOD and GPxs activity was decreased along with TAC in anemic patients (*P=۰.۰۲, **P=۰.۰۰۸, *P=۰.۰۳۸). Zinc and PAB levels correlated with some red blood cell indices. PAB was associated with anemia severity. Conclusion: Increased PAB and decreased zinc/selenium increased oxidant levels in anemic patients. RBC indices and anemia severity were correlated with oxidant/ antioxidant somewhere.

Keywords:

Anemia , Antioxidants , MIRN۱۲۲ , Oxidants , Selenium , Zinc.