BIOCHEMISTRY AND REGULATION OF L-LYSINE FERMENTATION

Because of the significance L-lysine economics, biochemical pathways of Llysine synthesis have been intensively investigated and elucidated, aiming inincreasing lysine production and reduction of production costs. Upon cellularabsorption, glucose is phosphorylated to glucose-6-hosphate with theconsumption of phosphoenolpyruvate (phosphotransferase system), whereas sucrose is converted into fructose and glucose-6-phosphate by the phosphotransferase system and invertase reaction. Glucose is catalyzed through the Embden-Meyerhof-Pamas (glycolysis) and pentose phosphatepathways. During glucose catabolism, glucose-6-phosphate isomerase (EC 5.3. 1.9) and glucose-6- phosphate dehydrogenase (EC 1.1. 14.9) compete for the substrate glucose-6-phosphate, resulting into either fructose- 6 phosphate or 6-phosphogluconolactone, respective

Keywords:

L-Lysine production , amino acids , corynebacteria , strain evelopment , mutagenesis , fermentation , downstream process , ion exchange chromatography , recombinant DNA , feed additive , regulation