Development of rapid and simultaneous detection of four major foodborne pathogens using a multiplex PCR method
Publish place: Trends in Pharmaceutical Sciences، Vol: 10، Issue: 2
Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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تاریخ نمایه سازی: 31 تیر 1403
Abstract:
Food borne diseases are an important public health problem has major impacts on human health, also affect trade and economic issues. Developing microbial cultures to detect foodborne pathogens is time-consuming and expensive. The aim of this study is to develop a multiplex (mPCR) method for the simultaneous detection of Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Salmonella enteritidis. Buffered peptone water (BPW) wasused as pre-enrichment. Simplex and multiplex PCR settings were optimized and applied to both pure co-cultures and artificially inoculated ready-to-eat food samples (falafel and chicken nugget). The four microorganisms could be detected individually and in enrichment media artificially inoculated at ۱۰۱ CFU/mL by mPCR.In conclusion, individual and combine growth of E. coli, S. enterica, S. aureus and, L. monocytogenes with low levels of contamination in the presence of food matrices such as falafel and chicken nuggets is effectively supported by BPW broth as co-culture medium before mPCR detection. The proposed protocol for pre-enrichment of E. Coli, S. Enterica, S. Aureus and, L. Monocytogenes in approximately ۳۴ hours. Compared to culture methods that require at least ۷ days. This significantly reduces analysis time, effort, and cost.
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Authors
Marzieh Rashedinia
Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz Iran
Aliakbar Rezaei
Food and Drug Administration, Shiraz University of Medical Sciences, Shiraz, Iran
Samaneh Bina
۱-Food and Drug Administration, Shiraz University of Medical Sciences, Shiraz, Iran ۲-Department of biology, Marvdasht branch, Islamic Azad University, Marvdasht, Iran
Mohammad Javad Raee
Center for Nanotechnology in Drug Delivery