Marieh Honarmand - Associate Professor, Oral and Dental Disease Research Center AND Department of Oral Medicine, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
Alireza Nakhaee - Professor, Cellular and Molecular Research Center AND Department of Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Vahid Okati - Dentist, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran

BACKGROUND AND AIM: Recurrent aphthous stomatitis (RAS) results due to a multiple of causes, amongst which stress is one of the most important factors. On the other hand, salivary alpha (α)-amylase (SAA) is a secretory protein that increases in stress conditions. This study evaluated SAA level in subjects with RAS. METHODS: In this case-control (descriptive-analytical) study, unstimulated saliva samples were collected from ۲۷ patients with RAS and ۲۹ healthy controls. SAA activity was determined by spectrophotometric method using commercially available kit according to manufacturer procedure. Data were analyzed using SPSS software with t test (P < ۰.۰۵ was considered significant). RESULTS: SAA level in patients with RAS was ۸۰.۷۸ ± ۴.۶۹ U/ml and ۶۵.۶۱ ± ۲۷.۵۲ U/ml during recurrence and recovery, respectively (P = ۰.۰۰۵). SAA level in control group was ۱۹.۹۹ ± ۴.۶۵ U/ml. There was a significant difference in the SAA level between RAS and control groups. CONCLUSION: SAA level has been increased in patients with aphthous ulcer during recurrence, which may indicate an association between aphthous ulcer and stress.

Aphthous Stomatitis, Salivary Alpha-Amylases, Stress