Siroos Tarighi - MSc, Department of Cellular and molecular biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Marjan marjaninia - MCs, Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Spiedeh khaleghi - Ph.D, Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Majid Sadeghizadeh - Ph.D, Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Maryam Montazeri - Assistant Professor, Department of Medical Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Background: It is essential to find natural compounds with wound-healing properties that are accompanied by antibacterial effects. Therefore, in the present study, the wound-healing potential of curcumin dendrosomes on fibroblast cell line was evaluated. Methods: The HFF۱ cell proliferation was evaluated by MTT test after ۴۸ hours of curcumin (Cur) and curcumin dendrosomes (Cur.den) treatments. Scratching test was used to check cell migration and the expression levels of TNF-α and TGF-β genes in cells were studied by Real-Time PCR technique. To investigate the antibacterial activity of Cur and Cur.den against S. epidermidis, well diffusion and microdilution tests were used. Results: Cur.den improved significantly HFF۱ cell proliferation and migration compared to both untreated control and Cur-treated cells. Moreover, this nanoformulation significantly overexpressed TGF-β and downregulated TNF-α genes compared to control cells. Both Cur and Cur.den reduced S. epidermidis growth in well diffusion assay and their MICs were measured at ۲۰۰ and ۱۰۰ µM, respectively. Conclusion: The Cur.den has a high potential to be used as a wound-healing drug. However, studies in animal models and clinical settings are recommended.

Curcumin, Dendrosome, Expression, Gene, Migration, Proliferation.