The association of sperm BAX and BCL-۲ gene expression with reproductive outcome in Oligoasthenoteratozoospermia cases undergoing intracytoplasmic sperm injection: A case-control study
Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJRM-22-6_005
تاریخ نمایه سازی: 17 شهریور 1403
Abstract:
Background: The B-cell lymphoma ۲ (BCL-۲) protein is one of the members of the BCL-۲ associated X (BAX) protein family that acts as an inducer of apoptosis.
Objective: The present study aims to investigate the association between BAX and BCL-۲ gene expression with reproductive outcome, in cases undergoing intracytoplasmic sperm injection.
Materials and Methods: In this case-control study, ۵۰ men were divided into the healthy fertile and oligoasthenoteratozoospermic infertile men (n = ۲۵/each). They were subjected to history taking, clinical examination, and semen analysis. Expression of BAX and BCL-۲ genes were measured using real-time polymerase chain reaction. The DNA fragmentation index was measured using the sperm chromatin dispersion assay technique. Using World Health Organization criteria, sperm parameters were evaluated.
Results: Evaluation of apoptosis-related genes showed that oligoasthenoteratozoospermic significantly increased mRNA expression of BAX, and significantly decreased mRNA expression of BCL-۲, when compared with control. Moreover, the BAX/BCL-۲ ratio was significantly higher in oligoasthenoteratozoospermic compared to the normozoospermic group (p = ۰.۰۱). Also, this study showed that the BAX and BCL-۲ genes expression had a significant correlation with sperm quality, and DNA fragmentation in the oligoasthenoteratozoospermic group (p = ۰.۰۱). The oligoasthenoteratozoospermic men, had a considerably lower proportion of fertilization rate and good-quality embryos at the cleavage stage than the normozoospermic subjects (p = ۰.۰۱). A significant correlation was observed between the expression of BAX and BCL-۲ genes, fertilization, and embryo quality (p = ۰.۰۱).
Conclusion: We concluded that the sperm BAX/BCL-۲ ratio demonstrates a significant correlation with fertilization rate and embryo quality.
Authors
Fatemeh Babaei
Biology Department, Islamic Azad University, Qom Branch, Qom, Iran.
Maryam Khoshsokhan Muzaffar
Biology Department, Islamic Azad University, Qom Branch, Qom, Iran.
Rahil Jannatifar
Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.
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