زهرا صلواتیها - Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
سیدجلال کیانی - Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
احمد توکلی - Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
محمدهادی کربلایی نیا - Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran, Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran
علیرضا جوان - Student Research Committee, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
زهرا صفایی - Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
محمدرضا رضوانی - Department of Hematology and Blood Transfusion, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
فرهاد بخارایی سلیم - Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
سیدحمیدرضا منوری - Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Introduction: Respiratory tract infection (RTI) is among the important factors that can threaten human health and is one of the most prevalent etiological agents of death, especially in young children. Viruses account for approximately ۸۰% of various RTIs worldwide. Our study aimed to investigate the molecular prevalence of seven respiratory viruses in RTI. Our results can help develop appropriate public health strategies and treatment management of RTIs. Materials and Methods: ۱۲۰ respiratory samples were obtained from patients with flu-like manifestations from hospitals referred to the Iran University of Medical Sciences during ۲۰۲۰-۲۰۲۲. Nucleic acid extraction was performed, and then real-time polymerase chain reaction  was performed to analyze the specimens to investigate seven respiratory viruses. Results: Of the ۱۲۰ respiratory specimens collected from patients with RTI, viruses were identified in ۴۱ (۳۴.۱۶%) samples, encompassing ۲۴ (۴۰.۷%) females and ۱۷ (۲۷.۹%) males. SARS-CoV-۲ (n = ۲۲, ۱۸.۳%) and Parainfluenza virus (n=۱۸, ۱۵%) were the predominant detected viruses in the present study, followed by respiratory syncytial viruses (n=۱, ۰.۸%). Other viruses like Metapneumovirus, Adenovirus, Bocavirus, and Rhinovirus were not found in our study. We could not detect any co-infection in our analysis. Conclusion: SARS-CoV-۲ and Parainfluenza-virus were the predominant viruses detected in our study, followed by respiratory syncytial viruses. Other viruses, such as Metapneumovirus, Adenovirus, Bocavirus, and Rhinovirus, have not been found in our study. We could not detect any co-infection in our consideration. This result can help develop appropriate public health strategies, targeting prevention and avoiding inappropriate treatment such as using unnecessary antibiotics.

Parainfluenza virus, Real-time PCR, Respiratory syncytial virus, Respiratory tract infection, SARS-CoV-۲, عفونت دستگاه تنفسی, ویروس سارس۲, ریل تایم پی سی آر, ویروس پاراآنفلوآنزا, ویروس سنسیشیال تنفسی