The Predictive Role of MicroRNA۱۹-b in Myocardial Infarction in Rat

Introduction: Cardiovascular disease is one of the leading causes of death worldwide. Acute myocardial infarction (AMI) is the most common and deadly disease of all types of cardiovascular disease. Many biomarkers such as troponin, CK-MB and various microRNAs have been shown to be involved in a variety of diseases, especially cardiovascular disease. Circulating MicroRNAs may be important and early biomarkers for the prognosis of cardiovascular disease. The aim of this study was to investigate the predictive role of miRNA-۱۹b as a potential biomarker for AMI.Materials and Methods: In this case control method we divided ۲۰ rats in Wistar breed (weight ۲۵۰-۳۰۰ g) into two groups of myocardial infarctions and healthy. Standard conditions such as a temperature of ۲۵ ° C, enough water and food were created in the animal room of Mashhad Medical School. ۱۵۰ mg of isoprenaline was dissolved in ۲ ml of injected distilled water and the solution was injected subcutaneously for two days. ۲۴ hours after the last injection, myocardial infarction was confirmed by assessment of (CK-MB and troponin).Results: Based on the findings, levels of troponin I and also CK-MB were higher in the group of rats induced myocardial infarction compared to healthy rats (P<۰.۰۰۱). In addition, the analyzes showed a significant up-regulation for miR-۱۹b expression in the Treat group (P<۰.۰۰۱).Conclusions: These findings indicate that circulating miRNAs, especially microRNA ۱۹b, could be excellent candidates as biomarkers for heart disease, especially EMI.

Keywords:

microRNAs , Cardiovascular Disease , Acute myocardial infarction , MicroRNA-۱۹b