Antitumor activity of exosomes derived from Toxoplasma infected cells on BALB/C mice induced with intestinal cancer

Publish Year: 1403
نوع سند: مقاله کنفرانسی
زبان: English
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ICGCS02_413

تاریخ نمایه سازی: 17 دی 1403

Abstract:

In recent years, cancer treatments based on pathogens have attracted a lot of attention. Infection by parasites such as Toxoplasma gondii and Plasmodium can inhibit tumor growth. Exosomes from host cells infected with Toxoplasma have an anti-infective effect. These exosomes convey the message of "infection" to the immune cells and stimulate them to Activate the safety response. Methods: We purchased the mouse colorectal cancer cell line (CT۲۶). The cell culture medium used in this experiment (RPMI) was supplemented with ۱۶۴۰ with ۱% penicillin-streptomycin solution and ۱۰% bovine embryo Serum (FBS). Cultures were incubated in ۵% CO۲ at ۳۷°C. The cultured cases were incubated in ۵% CO۲ at ۳۷ degrees Celsius. The Toxoplasma strain used in this study was the Me۴۹ T. gondii strain. Also, ۸-week-old BALB/c mice were used. The mice were divided into two groups and the Me۴۹ strain was injected subcutaneously into them. Then CT۲۶ cell suspension ۵x۱۰۶. T. gondii treatment of mice with Colorectal cancer After immunization of mice with T. gondii strain Me۴۹, mice were examined It was injected with ۵x۱۰۶ CT۲۶ cells. We performed a quantitative polymerase chain reaction (qPCR). Amplification of the ۵۲۹ bp target gene in T. gondii done. Flow cytometry analysis Mice with CRC were treated with T. gondii or exosomes were sacrificed before flow cytometry (FCM) analysis. Properties of exosomes We observed the size, morphology, and distribution of exosomes by transmission electron microscopy (TEM). Indirect immunofluorescence Exosomes were labeled by ExoSparker Exosome Membrane Treatment of colon cancer Mice with exosomes Female BALB/c mice (۶ weeks old) were randomly weighed. When it was a tumor It can be seen, the treatment was done according to the following Design: An intratumoral injection with ۱۰ mg of DC-exo (DC-exo group), ۱۰ mg DC-Me۴۹-exo (DC-Me۴۹-exo group), and ۱۰ ml PBS (PBS group). Two injections were performed on days ۱ and ۳ After visualization of the tumor. On the ۱۲th day after inoculation, Tumor progression was monitored using the IVIS spectrum Imaging system (IVIS Spectrum, USA). Statistical analysis All the data was collected using the software this Significant difference between the experimental and control groups. The group was analyzed using t-test or one-way ANOVA with Dunnett’s multiple comparisons. Result: Results showed that tumor growth was significantly inhibited after treatment with DC-Me۴۹-exo. The proportion of polymorphonuclear bone marrow-derived granulocyte suppressor cells (G-MDSCs, CD۱۱b+ Ly۶G+ and monocyte myeloid-derived suppressor cells (M-MDSCs, CD۱۱b+ Ly۶C+) decreased in the DC-Me۴۹-exo group compared to the control group in vitro and in vivo groups. The proportion of DCs (CD۴۵+ CD۱۱c+) increased significantly DC-Me۴۹-Exo group. The levels of interleukin-۶ (IL-۶) and granulocyte-macrophage colony-stimulating factor (GM-CSF) were significantly decreased after treatment with DC-Me۴۹-exo. Furthermore, we found that DC-Me۴۹-exo induced MDSCs mainly by Inhibition of the signal transducer and activator of the transcription (STAT۳) signaling pathway. Conclusion: Research has shown that infection with single-celled parasites T. gondii can inhibit tumor growth. In this study, we identified the Distribution of T. gondii in tumor-bearing mice, but without parasites found in tumor tissues

Authors

Farzin Samadi

Department of Microbiology and Parasitology, Kia Tashkhis Ayaz Laboratory, Urmia, Iran

Seyran Mohammad Pouri Naeim

Department of Microbiology and Parasitology, Kia Tashkhis Ayaz Laboratory, Urmia, Iran

Rozhin Golestani Siaraki

Department of Microbiology and Parasitology, Kia Tashkhis Ayaz Laboratory, Urmia, Iran

Sana Vahed Aghbolagh

Department of Microbiology and Parasitology, Kia Tashkhis Ayaz Laboratory, Urmia, Iran

Toran Ebrahimi

Department of Microbiology and Parasitology, Kia Tashkhis Ayaz Laboratory, Urmia, Iran

Mohammad Yousefzadeh

Department of Microbiology and Parasitology, Kia Tashkhis Ayaz Laboratory, Urmia, Iran