Molecular and Biochemical Approaches to Evaluating Cypermethrin Resistance in Aedes aegypti: Insights from ELISA, PCR, and Sanger Sequencing

Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
View: 40

This Paper With 20 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_JMCH-7-10_012

تاریخ نمایه سازی: 19 دی 1403

Abstract:

Dengue fever control using chemical methods is often performed by the community due to its immediate and effective results. Control is applied through thermal fogging. Continuous use of chemicals beyond the recommended dose leads to resistance. Methods to determine mosquito resistance include the World Health Organization (WHO) Susceptibility tests using impregnated paper, non-specific esterase enzyme biochemistry using Enzyme-linked immunosorbent assay (ELISA reader), and VGSC gene identification (Voltage Gated Sodium Channel) using PCR (Polymerase Chain Reaction). Kediri Regency has been using Cypermethrin insecticide for one year to prevent resistance. This study aims to predict the resistance of Aedes aegypti mosquitoes to Cypermethrin ۰.۰۵% insecticide. This is an observational laboratory study and efficacy test with a cross-sectional design. Third-generation (F۳) Aedes aegypti mosquitoes were used as research subjects, with Cypermethrin ۰.۰۵% as the insecticide. The study variable is the percentage of mosquitoes that die within ۶۰ minutes and ۲۴ hours post thermal fogging application. Efficacy tests and VGSC gene identification results show that up to the ۶th generation (R۳), mosquitoes remain susceptible to Cypermethrin ۰.۰۵%. The mortality percentage is ≥ ۹۸%, and the non-esterase absorbance value increases from ۰.۲۷۶ (R۰) to ۰.۴۸۸ (R۳). Further resistance testing using ELISA, PCR, and chromosome examination with the Sanger method continues until mosquitoes show resistance to Cypermethrin ۰.۰۵%.

Authors

Demes Nurmayanti

Department of Environmental Health, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia

Marlik Marlik

Department of Environmental Health, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia

Ngadino Ngadino

Department of Environmental Health, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia

Slamet Wardoyo

Department of Environmental Health, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • . Auliya I.N., Sulistio I., Pratiwi W.K., Study on the ...
  • . Auliya I.N., Sulistio I., Pratiwi W.K., Study on the ...
  • . Periatama S., Lestari R.M., Prasida D.W., Hubungan perilaku ۳m ...
  • . Sutriyawan A., Wirawati K., Suherdin S., Kejadian demam berdarah ...
  • . Prasetyowati H., Astuti E.P., Ruliansyah A., Penggunaan insektisida rumah ...
  • . Hendri J., Kusnandar A.J., Astuti E.P., Identifikasi jenis bahan ...
  • . Gusfa F., Aziz A.,Anwar T., Optimization of drying temperature ...
  • . Kurnia R., Yusuf M., Diansafitri M., Hardiatna T., Analisis ...
  • . Nurmayanti D., Ngadino N., Marlik M., Wardoyo S., Spatial ...
  • . Nurmayanti D., Resistance of aedes aegypti mosquito on cypermethrin ...
  • . Pradani F.Y., Ipa M., Yuliasih Y., Status resistensi aedes ...
  • . Sunaryo S., Widiastuti D., Resistensi aedes aegypti terhadap insektisida ...
  • . Mardihusodo S.J.M.S.J., Application of non-specific esterase enzyme microassays to ...
  • . Nurmayanti D., Marlik N., Conventional detection of resistance of ...
  • . Lima E.P., Paiva M.H.S., de Araújo A.P., da Silva ...
  • . Yuenleni Y., Langkah-langkah optimasi pcr, Indonesian Journal of Laboratory, ...
  • . Contreras-Perera Y., Ponce-Garcia G., Villanueva-Segura K., Lopez-Monroy B., Rodríguez-Sanchez ...
  • . Fernando H.S.D., Saavedra-Rodriguez K., Perera R., Black W.C., De ...
  • . de Araújo A.P., Paiva M.H.S., Cabral A.M., Cavalcanti A.E.H.D., ...
  • . Epelboin Y., Wang L., Giai Gianetto Q., Choumet V., ...
  • . Novita I.B., Martini M., Hestiningsih R., Yuliawati S., Kusariana ...
  • . Tomia A., Hadi U.K., Soviana S., Retnani E.B., December. ...
  • . Lubis Z.H., Nurfadly N., Resistance test of aedes aegypti ...
  • . Thompson A.J., Verdin P.S., Burton M.J., Davies T.E., Williamson ...
  • . Yuan H., Shan W., Zhang Y., Yan H., Li ...
  • . Amelia-Yap Z.H., Chen C.D., Sofian-Azirun M., Low V.L., Pyrethroid ...
  • . Bickersmith S.A., Jurczynski J.D., Sallum M.A.M., Chaves L.S., Bergo ...
  • . Najmi A., Sadasivam B., Ray A., How to choose ...
  • . Konan L.Y., Oumbouke W.A., Silué U.G., Coulibaly I.Z., Ziogba ...
  • . Bass C., Nauen R., The molecular mechanisms of insecticide ...
  • . Fang Y., Tambo E., Xue J.-B., Zhang Y., Zhou ...
  • . Moyes C.L., Vontas J., Martins A.J., Ng L.C., Koou ...
  • . Fang Y., Tambo E., Xue J.B., Zhang Y., Zhou ...
  • . Weill M., Lutfalla G., Mogensen K., Chandre F., Berthomieu ...
  • . Wolff G.H., Lahondère C., Vinauger C., Rylance E., Riffell ...
  • . Chandrasegaran K., Lahondère C., Escobar L.E., Vinauger C., Linking ...
  • . Salazar E., Gómez-Hernández E. and Ibarguen-Mondragón, E., July. Population ...
  • نمایش کامل مراجع